Efficient conversion of hemicellulose into high-value product and electric power by enzyme-engineered bacterial consortia

Bo Liang, Jing Yang, Chen-Fei Meng, Ya-Ru Zhang, Lu Wang, Li Zhang, Jia Liu, Zhen-Chao Li, Serge Cosnier (), Ai-Hua Liu () and Jian-Ming Yang ()
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Bo Liang: Qingdao Agricultural University
Jing Yang: Qingdao Agricultural University
Chen-Fei Meng: Qingdao Agricultural University
Ya-Ru Zhang: Qingdao University
Lu Wang: Qingdao Agricultural University
Li Zhang: Qingdao Agricultural University
Jia Liu: Qingdao Agricultural University
Zhen-Chao Li: Qingdao University
Serge Cosnier: 44-100
Ai-Hua Liu: Qingdao University
Jian-Ming Yang: Qingdao Agricultural University

Nature Communications, 2024, vol. 15, issue 1, 1-13

Abstract: Abstract As an abundant agricultural and forestry biomass resource, hemicelluloses are hard to be effectively degraded and utilized by microorganisms due to the constraints of membrane and metabolic regulations. Herein, we report a synthetic extracellular metabolic pathway with hemicellulose-degrading-enzymes controllably displayed on Escherichia coli surface as engineered bacterial consortia members for efficient utilization of xylan, the most abundant component in hemicellulose. Further, we develop a hemicellulose/O2 microbial fuel cell (MFC) configuring of enzyme-engineered bacterial consortia based bioanode and bacterial-displayed laccase based biocathode. The optimized MFC exhibited an open-circuit voltage of 0.71 V and a maximum power density (Pmax) of 174.33 ± 4.56 µW cm−2. Meanwhile, 46.6% (w/w) α-ketoglutarate was produced in this hemicellulose fed-MFC. Besides, the MFC retained over 95% of the Pmax during 6 days’ operation. Therefore, this work establishes an effective and sustainable one-pot process for catalyzing renewable biomass into high-value products and electricity in an environmentally-friendly way.

Date: 2024
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DOI: 10.1038/s41467-024-53129-0

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