Noninvasive in vivo photoacoustic detection of malaria with Cytophone in Cameroon

Aayire C. Yadem, Jillian N. Armstrong, Mustafa Sarimollaoglu, Civian Kiki Massa, Jean-Michel Ndifo, Yulian A. Menyaev, Anastasie Mbe, Kacey Richards, Martina Wade, Yushun Zeng, Ruimin Chen, Qifa Zhou, Elvis Meten, Rodrigue Ntone, Yves Le Grand Napa Tchuedji, Safi Ullah, Ekaterina I. Galanzha, Lucrèce Eteki, Hortense Kamga Gonsu, Alexandru Biris, James Y. Suen, Yap Boum, Vladimir P. Zharov () and Sunil Parikh ()
Additional contact information
Aayire C. Yadem: Bioventures/UAMS
Jillian N. Armstrong: Yale School of Public Health
Mustafa Sarimollaoglu: Bioventures/UAMS
Civian Kiki Massa: Epicentre
Jean-Michel Ndifo: University of Yaoundé I
Yulian A. Menyaev: Bioventures/UAMS
Anastasie Mbe: Epicentre
Kacey Richards: Yale School of Public Health
Martina Wade: Yale School of Public Health
Yushun Zeng: University of Southern California
Ruimin Chen: University of Southern California
Qifa Zhou: University of Southern California
Elvis Meten: Epicentre
Rodrigue Ntone: Epicentre
Yves Le Grand Napa Tchuedji: University of Yaoundé I
Safi Ullah: Bioventures/UAMS
Ekaterina I. Galanzha: Bioventures/UAMS
Lucrèce Eteki: Epicentre
Hortense Kamga Gonsu: University of Yaoundé I
Alexandru Biris: University of Arkansas at Little Rock (UALR)
James Y. Suen: Bioventures/UAMS
Yap Boum: Epicentre
Vladimir P. Zharov: Bioventures/UAMS
Sunil Parikh: Yale School of Public Health

Nature Communications, 2024, vol. 15, issue 1, 1-15

Abstract: Abstract Current malaria diagnostics are invasive, lack sensitivity, and rapid tests are plagued by deletions in target antigens. Here we introduce the Cytophone, an innovative photoacoustic flow cytometer platform with high-pulse-rate lasers and a focused ultrasound transducer array to noninvasively detect and identify malaria-infected red blood cells (iRBCs) using specific wave shapes, widths, and time delays generated from the absorbance of laser energy by hemozoin, a universal biomarker of malaria infection. In a population of Cameroonian adults with uncomplicated malaria, we assess our device for safety in a cross-sectional cohort (n = 10) and conduct a performance assessment in a longitudinal cohort (n = 20) followed for 30 ± 7 days after clearance of parasitemia. Longitudinal cytophone measurements are compared to point-of-care and molecular assays (n = 94). Cytophone is safe with 90% sensitivity, 69% specificity, and a receiver-operator-curve-area-under-the-curve (ROC-AUC) of 0.84, as compared to microscopy. ROC-AUCs of Cytophone, microscopy, and RDT compared to quantitative PCR are not statistically different from one another. The ability to noninvasively detect iRBCs in the bloodstream is a major advancement which offers the potential to rapidly identify both the large asymptomatic reservoir of infection, as well as diagnose symptomatic cases without the need for a blood sample.

Date: 2024
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-024-53243-z Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-53243-z

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-024-53243-z

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().