Structuring lipid nanoparticles, DNA, and protein corona into stealth bionanoarchitectures for in vivo gene delivery

Serena Renzi, Luca Digiacomo, Daniela Pozzi, Erica Quagliarini, Elisabetta Vulpis, Maria Valeria Giuli, Angelica Mancusi, Bianca Natiello, Maria Gemma Pignataro, Gianluca Canettieri, Laura Magno, Luca Pesce, Valentina Lorenzi, Samuele Ghignoli, Luisa Loconte, Carmela Maria Montone, Anna Laura Capriotti, Aldo Laganà, Carmine Nicoletti, Heinz Amenitsch, Marco Rossi, Francesco Mura, Giacomo Parisi, Francesco Cardarelli, Alessandra Zingoni (), Saula Checquolo () and Giulio Caracciolo ()
Additional contact information
Serena Renzi: Sapienza University of Rome
Luca Digiacomo: Sapienza University of Rome
Daniela Pozzi: Sapienza University of Rome
Erica Quagliarini: Sapienza University of Rome
Elisabetta Vulpis: Sapienza University of Rome
Maria Valeria Giuli: Laboratory affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti
Angelica Mancusi: Sapienza University of Rome
Bianca Natiello: Sapienza University of Rome
Maria Gemma Pignataro: Sapienza University of Rome
Gianluca Canettieri: Sapienza University of Rome
Laura Magno: Sapienza University of Rome
Luca Pesce: Scuola Normale Superiore
Valentina Lorenzi: Scuola Normale Superiore
Samuele Ghignoli: Scuola Normale Superiore
Luisa Loconte: Sapienza University of Rome
Carmela Maria Montone: Sapienza University of Rome
Anna Laura Capriotti: Sapienza University of Rome
Aldo Laganà: Sapienza University of Rome
Carmine Nicoletti: Sapienza University of Rome
Heinz Amenitsch: Graz University of Technology
Marco Rossi: Sapienza University of Rome
Francesco Mura: Sapienza University of Rome
Giacomo Parisi: Sapienza University of Rome
Francesco Cardarelli: Scuola Normale Superiore
Alessandra Zingoni: Sapienza University of Rome
Saula Checquolo: Laboratory affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti
Giulio Caracciolo: Sapienza University of Rome

Nature Communications, 2024, vol. 15, issue 1, 1-20

Abstract: Abstract Lipid nanoparticles (LNPs) play a crucial role in addressing genetic disorders, and cancer, and combating pandemics such as COVID-19 and its variants. Yet, the ability of LNPs to effectively encapsulate large-size DNA molecules remains elusive. This is a significant limitation, as the successful delivery of large-size DNA holds immense potential for gene therapy. To address this gap, the present study focuses on the design of PEGylated LNPs, incorporating large-sized DNA, departing from traditional RNA and ionizable lipids. The resultant LNPs demonstrate a unique particle morphology. These particles were further engineered with a DNA coating and plasma proteins. This multicomponent bionanoconstruct exhibits enhanced transfection efficiency and safety in controlled laboratory settings and improved immune system evasion in in vivo tests. These findings provide valuable insights for the design and development of bionanoarchitectures for large-size DNA delivery, opening new avenues for transformative gene therapies.

Date: 2024
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-024-53569-8 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-53569-8

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-024-53569-8

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().