Ultra-inert lanthanide chelates as mass tags for multiplexed bioanalysis

Tomáš David, Miroslava Šedinová, Aneta Myšková, Jaroslav Kuneš, Lenka Maletínská, Radek Pohl, Martin Dračínský, Helena Mertlíková-Kaiserová, Karel Čížek, Blanka Klepetářová, Miroslava Litecká, Antonín Kaňa, David Sýkora, Adam Jaroš, Michal Straka and Miloslav Polasek ()
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Tomáš David: Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences
Miroslava Šedinová: Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences
Aneta Myšková: Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences
Jaroslav Kuneš: Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences
Lenka Maletínská: Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences
Radek Pohl: Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences
Martin Dračínský: Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences
Helena Mertlíková-Kaiserová: Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences
Karel Čížek: Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences
Blanka Klepetářová: Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences
Miroslava Litecká: Institute of Inorganic Chemistry, Czech Academy of Sciences
Antonín Kaňa: University of Chemistry and Technology Prague
David Sýkora: University of Chemistry and Technology Prague
Adam Jaroš: Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences
Michal Straka: Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences
Miloslav Polasek: Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences

Nature Communications, 2024, vol. 15, issue 1, 1-12

Abstract: Abstract Coordination compounds of lanthanides are indispensable in biomedical applications as MRI contrast agents and radiotherapeutics. However, since the introduction of the chelator DOTA four decades ago, there has been only limited progress on improving their thermodynamic stability and kinetic inertness, which are essential for safe in vivo use. Here, we present ClickZip, an innovative synthetic strategy employing a coordination-templated formation of a 1,5-triazole bridge that improves kinetic inertness up to a million-fold relative to DOTA, expanding utility of lanthanide chelates beyond traditional uses. Acting as unique mass tags, the ClickZip chelates can be released from (biological) samples by acidic hydrolysis, chromatographically distinguished from interfering lanthanide species, and sensitively detected by mass spectrometry. Lanthanides enclosed in ClickZip chelates are chemically almost indistinguishable, providing a more versatile alternative to chemically identical isotopic labels for multiplexed analysis. The bioanalytical potential is demonstrated on tagged cell-penetrating peptides in vitro, and anti-obesity prolactin-releasing peptides in vivo.

Date: 2024
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DOI: 10.1038/s41467-024-53867-1

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