The role of the essential GTPase ObgE in regulating lipopolysaccharide synthesis in Escherichia coli

Liselot Dewachter (), Babette Deckers, Israel Mares-Mejía, Elen Louwagie, Silke Vercauteren, Paul Matthay, Simon Brückner, Anna-Maria Möller, Franz Narberhaus, Sibylle C. Vonesch, Wim Versées and Jan Michiels
Additional contact information
Liselot Dewachter: KU Leuven
Babette Deckers: Vrije Universiteit Brussel
Israel Mares-Mejía: Vrije Universiteit Brussel
Elen Louwagie: KU Leuven
Silke Vercauteren: KU Leuven
Paul Matthay: KU Leuven
Simon Brückner: Ruhr University Bochum
Anna-Maria Möller: Ruhr University Bochum
Franz Narberhaus: Ruhr University Bochum
Sibylle C. Vonesch: KU Leuven
Wim Versées: Vrije Universiteit Brussel
Jan Michiels: KU Leuven

Nature Communications, 2024, vol. 15, issue 1, 1-19

Abstract: Abstract During growth, cells need to synthesize and expand their envelope, a process that requires careful regulation. Here, we show that the GTPase ObgE of E. coli contributes to the regulation of lipopolysaccharide (LPS) synthesis, an essential component of the Gram-negative outer membrane. Using a dominant-negative mutant (named ‘ObgE*’), we show a direct interaction between ObgE and LpxA, which catalyzes the first step in LPS synthesis. This interaction is enhanced by the mutation in ObgE* which, when bound to GTP, leads to inhibition of LpxA, decreased LPS synthesis, and cell death. Although wild-type ObgE does not exert the same strong effects as ObgE* on LpxA or LPS synthesis, our data indicate that ObgE participates in the regulation of cell envelope synthesis in E. coli. Because ObgE also influences other cellular functions (i.e., ribosome assembly, DNA replication, etc.), it seems increasingly plausible that this GTPase coordinates several processes to finetune cell growth.

Date: 2024
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-024-53980-1 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-53980-1

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-024-53980-1

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().