Non-targeted N-glycome profiling reveals multiple layers of organ-specific diversity in mice

Johannes Helm, Stefan Mereiter, Tiago Oliveira, Anna Gattinger, David M. Markovitz, Josef M. Penninger, Friedrich Altmann and Johannes Stadlmann ()
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Johannes Helm: Muthgasse 18
Stefan Mereiter: Spitalgasse 23
Tiago Oliveira: Spitalgasse 23
Anna Gattinger: Dr. Bohr-Gasse 3
David M. Markovitz: University of Michigan
Josef M. Penninger: Spitalgasse 23
Friedrich Altmann: Muthgasse 18
Johannes Stadlmann: Muthgasse 18

Nature Communications, 2024, vol. 15, issue 1, 1-16

Abstract: Abstract N-glycosylation is one of the most common protein modifications in eukaryotes, with immense importance at the molecular, cellular, and organismal level. Accurate and reliable N-glycan analysis is essential to obtain a systems-wide understanding of fundamental biological processes. Due to the structural complexity of glycans, their analysis is still highly challenging. Here we make publicly available a consistent N-glycome dataset of 20 different mouse tissues and demonstrate a multimodal data analysis workflow that allows for unprecedented depth and coverage of N-glycome features. This highly scalable, LC-MS/MS data-driven method integrates the automated identification of N-glycan spectra, the application of non-targeted N-glycome profiling strategies and the isomer-sensitive analysis of glycan structures. Our delineation of critical sub-structural determinants and glycan isomers across the mouse N-glycome uncovered tissue-specific glycosylation patterns, the expression of non-canonical N-glycan structures and highlights multiple layers of N-glycome complexity that derive from organ-specific regulations of glycobiological pathways.

Date: 2024
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DOI: 10.1038/s41467-024-54134-z

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