MatP local enrichment delays segregation independently of tetramer formation and septal anchoring in Vibrio cholerae

Elena Espinosa, Jihane Challita, Jean-Michel Desfontaines, Christophe Possoz, Marie-Eve Val, Didier Mazel, Martial Marbouty, Romain Koszul, Elisa Galli () and François-Xavier Barre ()
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Elena Espinosa: 1 Avenue de la Terrasse
Jihane Challita: 1 Avenue de la Terrasse
Jean-Michel Desfontaines: 1 Avenue de la Terrasse
Christophe Possoz: 1 Avenue de la Terrasse
Marie-Eve Val: Unité Plasticité du Génome Bactérien
Didier Mazel: Unité Plasticité du Génome Bactérien
Martial Marbouty: Unité Spatial Regulation of Genomes
Romain Koszul: Unité Spatial Regulation of Genomes
Elisa Galli: 1 Avenue de la Terrasse
François-Xavier Barre: 1 Avenue de la Terrasse

Nature Communications, 2024, vol. 15, issue 1, 1-14

Abstract: Abstract Vibrio cholerae harbours a primary chromosome derived from the monochromosomal ancestor of the Vibrionales (ChrI) and a secondary chromosome derived from a megaplasmid (ChrII). The coordinated segregation of the replication terminus of both chromosomes (TerI and TerII) determines when and where cell division occurs. ChrI encodes a homologue of Escherichia coli MatP, a protein that binds to a DNA motif (matS) that is overrepresented in replication termini. Here, we use a combination of deep sequencing and fluorescence microscopy techniques to show that V. cholerae MatP structures TerI and TerII into macrodomains, targets them to mid-cell during replication, and delays their segregation, thus supporting that ChrII behaves as a bona fide chromosome. We further show that the extent of the segregation delay mediated by MatP depends on the number and local density of matS sites, and is independent of its assembly into tetramers and any interaction with the divisome, in contrast to what has been previously observed in E. coli.

Date: 2024
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DOI: 10.1038/s41467-024-54195-0

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