Structural mechanism of CB1R binding to peripheral and biased inverse agonists

Punita Kumari, Szabolcs Dvorácskó, Michael D. Enos, Karthik Ramesh, Darrix Lim, Sergio A. Hassan, George Kunos, Resat Cinar, Malliga R. Iyer () and Daniel M. Rosenbaum ()
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Punita Kumari: The University of Texas Southwestern Medical Center
Szabolcs Dvorácskó: National Institutes of Health
Michael D. Enos: The University of Texas Southwestern Medical Center
Karthik Ramesh: The University of Texas Southwestern Medical Center
Darrix Lim: The University of Texas Southwestern Medical Center
Sergio A. Hassan: National Institutes of Health
George Kunos: National Institutes of Health
Resat Cinar: National Institutes of Health
Malliga R. Iyer: National Institutes of Health
Daniel M. Rosenbaum: The University of Texas Southwestern Medical Center

Nature Communications, 2024, vol. 15, issue 1, 1-13

Abstract: Abstract The cannabinoid receptor 1 (CB1R) regulates synaptic transmission in the central nervous system, but also has important roles in the peripheral organs controlling cellular metabolism. While earlier generations of brain penetrant CB1R antagonists advanced to the clinic for their effective treatment of obesity, such molecules were ultimately shown to exhibit negative effects on central reward pathways that thwarted their further therapeutic development. The peripherally restricted CB1R inverse agonists MRI-1867 and MRI-1891 represent a new generation of compounds that retain the metabolic benefits of CB1R inhibitors while sparing the negative psychiatric effects. To understand the mechanism of binding and inhibition of CB1R by peripherally restricted antagonists, we developed a nanobody/fusion protein strategy for high-resolution cryo-EM structure determination of the GPCR inactive state, and used this method to determine structures of CB1R bound to either MRI-1867 or MRI-1891. These structures reveal how these compounds retain high affinity and specificity for CB1R’s hydrophobic orthosteric site despite incorporating polar functionalities that lead to peripheral restriction. Further, the structure of the MRI-1891 complex along with accompanying molecular dynamics simulations shows how differential engagement with transmembrane helices and the proximal N-terminus can propagate through the receptor to contribute to biased inhibition of β-arrestin signaling.

Date: 2024
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DOI: 10.1038/s41467-024-54206-0

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