A modular platform for bioluminescent RNA tracking

Lila P. Halbers, Kyle H. Cole, Kevin K. Ng, Erin B. Fuller, Christelle E. T. Chan, Chelsea Callicoatte, Mariajose Metcalfe, Claire C. Chen, Ahfnan A. Barhoosh, Edison Reid-McLaughlin, Alexandra D. Kent, Zachary R. Torrey, Oswald Steward (), Andrej Lupták () and Jennifer A. Prescher ()
Additional contact information
Lila P. Halbers: University of California, Irvine
Kyle H. Cole: University of California, Irvine
Kevin K. Ng: University of California, Irvine
Erin B. Fuller: University of California, Irvine
Christelle E. T. Chan: University of California, Irvine
Chelsea Callicoatte: University of California, Irvine
Mariajose Metcalfe: University of California, Irvine
Claire C. Chen: University of California, Irvine
Ahfnan A. Barhoosh: University of California, Irvine
Edison Reid-McLaughlin: University of California, Irvine
Alexandra D. Kent: University of California, Irvine
Zachary R. Torrey: University of California, Irvine
Oswald Steward: University of California, Irvine
Andrej Lupták: University of California, Irvine
Jennifer A. Prescher: University of California, Irvine

Nature Communications, 2024, vol. 15, issue 1, 1-12

Abstract: Abstract A complete understanding of RNA biology requires methods for tracking transcripts in vivo. Common strategies rely on fluorogenic probes that are limited in sensitivity, dynamic range, and depth of interrogation, owing to their need for excitation light and tissue autofluorescence. To overcome these challenges, we report a bioluminescent platform for serial imaging of RNAs. The RNA tags are engineered to recruit light-emitting luciferase fragments (termed RNA lanterns) upon transcription. Robust photon production is observed for RNA targets both in cells and in live animals. Importantly, only a single copy of the tag is necessary for sensitive detection, in sharp contrast to fluorescent platforms requiring multiple repeats. Overall, this work provides a foundational platform for visualizing RNA dynamics from the micro to the macro scale.

Date: 2024
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DOI: 10.1038/s41467-024-54263-5

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