Reprogramming of flagellin receptor responses with surrogate ligands

Du-Hwa Lee (), Ho-Seok Lee, Min-Soo Choi, Katarzyna Parys, Kaori Honda, Yasumitsu Kondoh, Jung-Min Lee, Natalie Edelbacher, Geon Heo, Balaji Enugutti, Hiroyuki Osada, Ken Shirasu and Youssef Belkhadir ()
Additional contact information
Du-Hwa Lee: Dr. Bohr-Gasse 3
Ho-Seok Lee: Dr. Bohr-Gasse 3
Min-Soo Choi: Dr. Bohr-Gasse 3
Katarzyna Parys: Dr. Bohr-Gasse 3
Kaori Honda: Wako
Yasumitsu Kondoh: Wako
Jung-Min Lee: Dr. Bohr-Gasse 3
Natalie Edelbacher: Dr. Bohr-Gasse 3
Geon Heo: Dr. Bohr-Gasse 3
Balaji Enugutti: Dr. Bohr-Gasse 3
Hiroyuki Osada: Wako
Ken Shirasu: RIKEN Center for Sustainable Resource Science
Youssef Belkhadir: Dr. Bohr-Gasse 3

Nature Communications, 2024, vol. 15, issue 1, 1-14

Abstract: Abstract Receptor kinase (RK) families process information from small molecules, short peptides, or glycan ligands to regulate core cellular pathways in plants. To date, whether individual plant RKs are capable of processing signals from distinct types of ligands remains largely unexplored. Addressing this requires the discovery of structurally unrelated ligands that engage the same receptor. Here, we focus on FLAGELLIN-SENSING 2 (FLS2), an RK that senses a peptide of bacterial flagellin to activate antibacterial immunity in Arabidopsis. We interrogate >20,000 potential interactions between small molecules and the sensory domain of FLS2 using a large-scale reverse chemical screen. We discover two small molecules that interact with FLS2 in atypical ways. The surrogate ligands weakly activate the receptor to drive a functional antibacterial response channeled via unusual gene expression programs. Thus, chemical probes acting as biased ligands can be exploited to discover unexpected levels of output flexibility in RKs signal transduction.

Date: 2024
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-024-54271-5 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-54271-5

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-024-54271-5

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().