Structural effects of high laser power densities on an early bacteriorhodopsin photocycle intermediate

Quentin Bertrand, Przemyslaw Nogly, Eriko Nango, Demet Kekilli, Georgii Khusainov, Antonia Furrer, Daniel James, Florian Dworkowski, Petr Skopintsev, Sandra Mous, Isabelle Martiel, Per Börjesson, Giorgia Ortolani, Chia-Ying Huang, Michal Kepa, Dmitry Ozerov, Steffen Brünle, Valerie Panneels, Tomoyuki Tanaka, Rie Tanaka, Kensuke Tono, Shigeki Owada, Philip J. M. Johnson, Karol Nass, Gregor Knopp, Claudio Cirelli, Christopher Milne, Gebhard Schertler, So Iwata, Richard Neutze, Tobias Weinert () and Jörg Standfuss ()
Additional contact information
Quentin Bertrand: Paul Scherrer Institut
Przemyslaw Nogly: Paul Scherrer Institut
Eriko Nango: Sayo-gun
Demet Kekilli: Paul Scherrer Institut
Georgii Khusainov: Paul Scherrer Institut
Antonia Furrer: Paul Scherrer Institut
Daniel James: Paul Scherrer Institut
Florian Dworkowski: Paul Scherrer Institut
Petr Skopintsev: Paul Scherrer Institut
Sandra Mous: Paul Scherrer Institut
Isabelle Martiel: Paul Scherrer Institut
Per Börjesson: Box 462
Giorgia Ortolani: Box 462
Chia-Ying Huang: Paul Scherrer Institut
Michal Kepa: Paul Scherrer Institut
Dmitry Ozerov: Paul Scherrer Institut
Steffen Brünle: Paul Scherrer Institut
Valerie Panneels: Paul Scherrer Institut
Tomoyuki Tanaka: Sayo-gun
Rie Tanaka: Sayo-gun
Kensuke Tono: Sayo-gun
Shigeki Owada: Sayo-gun
Philip J. M. Johnson: Paul Scherrer Institut
Karol Nass: Paul Scherrer Institut
Gregor Knopp: Paul Scherrer Institut
Claudio Cirelli: Paul Scherrer Institut
Christopher Milne: Paul Scherrer Institut
Gebhard Schertler: Paul Scherrer Institut
So Iwata: Sakyo-ku
Richard Neutze: Box 462
Tobias Weinert: Paul Scherrer Institut
Jörg Standfuss: Paul Scherrer Institut

Nature Communications, 2024, vol. 15, issue 1, 1-11

Abstract: Abstract Time-resolved serial crystallography at X-ray Free Electron Lasers offers the opportunity to observe ultrafast photochemical reactions at the atomic level. The technique has yielded exciting molecular insights into various biological processes including light sensing and photochemical energy conversion. However, to achieve sufficient levels of activation within an optically dense crystal, high laser power densities are often used, which has led to an ongoing debate to which extent photodamage may compromise interpretation of the results. Here we compare time-resolved serial crystallographic data of the bacteriorhodopsin K-intermediate collected at laser power densities ranging from 0.04 to 2493 GW/cm2 and follow energy dissipation of the absorbed photons logarithmically from picoseconds to milliseconds. Although the effects of high laser power densities on the overall structure are small, in the upper excitation range we observe significant changes in retinal conformation and increased heating of the functionally critical counterion cluster. We compare light-activation within crystals to that in solution and discuss the impact of the observed changes on bacteriorhodopsin biology.

Date: 2024
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-024-54422-8 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-54422-8

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-024-54422-8

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().