Regeneration in the absence of canonical neoblasts in an early branching flatworm

Ludwik Gąsiorowski, Chew Chai, Andrei Rozanski, Gargi Purandare, Fruzsina Ficze, Athanasia Mizi, Bo Wang and Jochen C. Rink ()
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Ludwik Gąsiorowski: Max Planck Institute for Multidisciplinary Sciences
Chew Chai: Stanford University
Andrei Rozanski: Max Planck Institute for Multidisciplinary Sciences
Gargi Purandare: Max Planck Institute for Multidisciplinary Sciences
Fruzsina Ficze: Max Planck Institute for Multidisciplinary Sciences
Athanasia Mizi: University Medical Centre Göttingen
Bo Wang: Stanford University
Jochen C. Rink: Max Planck Institute for Multidisciplinary Sciences

Nature Communications, 2025, vol. 16, issue 1, 1-14

Abstract: Abstract The remarkable regenerative abilities of flatworms are closely linked to neoblasts – adult pluripotent stem cells that are the only division-competent cell type outside of the reproductive system. Although the presence of neoblast-like cells and whole-body regeneration in other animals has led to the idea that these features may represent the ancestral metazoan state, the evolutionary origin of both remains unclear. Here we show that the catenulid Stenostomum brevipharyngium, a member of the earliest-branching flatworm lineage, lacks conventional neoblasts despite being capable of whole-body regeneration and asexual reproduction. Using a combination of single-nuclei transcriptomics, in situ gene expression analysis, and functional experiments, we find that cell divisions are not restricted to a single cell type and are associated with multiple fully differentiated somatic tissues. Furthermore, the cohort of germline multipotency genes, which are considered canonical neoblast markers, are not expressed in dividing cells, but in the germline instead, and we experimentally show that they are neither necessary for proliferation nor regeneration. Overall, our results challenge the notion that canonical neoblasts are necessary for flatworm regeneration and open up the possibility that neoblast-like cells may have evolved convergently in different animals, independent of their regenerative capacity.

Date: 2025
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DOI: 10.1038/s41467-024-54716-x

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