Single-molecule two- and three-colour FRET studies reveal a transition state in SNARE disassembly by NSF

Sudheer K. Cheppali, Chang Li, Wenjing Xing, Ruirui Sun, Mengyi Yang, Yi Xue, Si-Yao Lu, Jun Yao, Shan Sun (), Chunlai Chen () and Sen-Fang Sui ()
Additional contact information
Sudheer K. Cheppali: Tsinghua University
Chang Li: Tsinghua University
Wenjing Xing: Tsinghua University
Ruirui Sun: Tsinghua University
Mengyi Yang: Tsinghua University
Yi Xue: Tsinghua University
Si-Yao Lu: Tsinghua University
Jun Yao: Tsinghua University
Shan Sun: Tsinghua University
Chunlai Chen: Tsinghua University
Sen-Fang Sui: Tsinghua University

Nature Communications, 2025, vol. 16, issue 1, 1-10

Abstract: Abstract SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptor) proteins are the minimal machinery required for vesicle fusion in eukaryotes. Formation of a highly stable four-helix bundle consisting of SNARE motif of these proteins, drives vesicle/membrane fusion involved in several physiological processes such as neurotransmission. Recycling/disassembly of the protein machinery involved in membrane fusion is essential and is facilitated by an AAA+ ATPase, N-ethylmaleimide sensitive factor (NSF) in the presence of an adapter protein, α-SNAP. Here we use single-molecule fluorescence spectroscopy approaches to elucidate the chain of events that occur during the disassembly of SNARE complex by NSF. Our observations indicate two major pathways leading to the sequential disassembly of the SNARE complex: one where a syntaxin separated intermediate state is observed before syntaxin disassembles first, and a second where Vamp disassembles from the other proteins first. These studies uncover two parallel sequential pathways for the SNARE disassembly by NSF along with a syntaxin separated intermediate that couldn’t be observed otherwise.

Date: 2025
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-024-55531-0 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-024-55531-0

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-024-55531-0

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().