NHSL3 controls single and collective cell migration through two distinct mechanisms

Nikita M. Novikov, Jinmei Gao, Artem I. Fokin, Nathalie Rocques, Giovanni Chiappetta, Karina D. Rysenkova, Diego Javier Zea, Anna Polesskaya, Joelle Vinh, Raphael Guerois and Alexis M. Gautreau ()
Additional contact information
Nikita M. Novikov: Institut Polytechnique de Paris
Jinmei Gao: Institute for Integrative Biology of the Cell (I2BC)
Artem I. Fokin: Institut Polytechnique de Paris
Nathalie Rocques: Institut Polytechnique de Paris
Giovanni Chiappetta: LPC CNRS UMR8249
Karina D. Rysenkova: Institut Polytechnique de Paris
Diego Javier Zea: Institute for Integrative Biology of the Cell (I2BC)
Anna Polesskaya: Institut Polytechnique de Paris
Joelle Vinh: LPC CNRS UMR8249
Raphael Guerois: Institute for Integrative Biology of the Cell (I2BC)
Alexis M. Gautreau: Institut Polytechnique de Paris

Nature Communications, 2025, vol. 16, issue 1, 1-16

Abstract: Abstract The molecular mechanisms underlying cell migration remain incompletely understood. Here, we show that knock-out cells for NHSL3, the most recently identified member of the Nance-Horan Syndrome family, are more persistent than parental cells in single cell migration, but that, in wound healing, follower cells are impaired in their ability to follow leader cells. The NHSL3 locus encodes several isoforms. We identify the partner repertoire of each isoform using proteomics and predict direct partners and their binding sites using an AlphaFold2-based pipeline. Rescue with specific isoforms, and lack of rescue when relevant binding sites are mutated, establish that the interaction of a long isoform with MENA/VASP proteins is critical at cell-cell junctions for collective migration, while the interaction of a short one with 14-3-3θ in lamellipodia is critical for single cell migration. Taken together, these results demonstrate that NHSL3 regulates single and collective cell migration through distinct mechanisms.

Date: 2025
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-024-55647-3 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-024-55647-3

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-024-55647-3

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().