Zika virus NS1 drives tunneling nanotube formation for mitochondrial transfer and stealth transmission in trophoblasts

Rafael T. Michita, Long B. Tran, Steven J. Bark, Deepak Kumar, Shay A. Toner, Joyce Jose, Indira U. Mysorekar () and Anoop Narayanan ()
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Rafael T. Michita: Baylor College of Medicine
Long B. Tran: Baylor College of Medicine
Steven J. Bark: Baylor College of Medicine
Deepak Kumar: Baylor College of Medicine
Shay A. Toner: University Park
Joyce Jose: University Park
Indira U. Mysorekar: Baylor College of Medicine
Anoop Narayanan: University Park

Nature Communications, 2025, vol. 16, issue 1, 1-19

Abstract: Abstract Zika virus (ZIKV) is unique among orthoflaviviruses in its vertical transmission capacity in humans, yet the underlying mechanisms remain incompletely understood. Here, we show that ZIKV induces tunneling nanotubes (TNTs) in placental trophoblasts which facilitate transfer of viral particles, proteins, mitochondria, and RNA to neighboring uninfected cells. TNT formation is driven exclusively via ZIKV non-structural protein 1 (NS1). Specifically, the N-terminal 1-50 amino acids of membrane-bound ZIKV NS1 are necessary for triggering TNT formation in host cells. Trophoblasts infected with TNT-deficient ZIKVΔTNT mutant virus elicited a robust antiviral IFN-λ 1/2/3 response relative to WT ZIKV, suggesting TNT-mediated trafficking allows ZIKV cell-to-cell transmission camouflaged from host defenses. Using affinity purification-mass spectrometry of cells expressing wild-type NS1 or non-TNT forming NS1, we found mitochondrial proteins are dominant NS1-interacting partners. We demonstrate that ZIKV infection or NS1 expression induces elevated mitochondria levels in trophoblasts and that mitochondria are siphoned via TNTs from healthy to ZIKV-infected cells. Together our findings identify a stealth mechanism that ZIKV employs for intercellular spread among placental trophoblasts, evasion of antiviral interferon response, and the hijacking of mitochondria to augment its propagation and survival and offers a basis for novel therapeutic developments targeting these interactions to limit ZIKV dissemination.

Date: 2025
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DOI: 10.1038/s41467-025-56927-2

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