Medium from human iPSC-derived primitive macrophages promotes adult cardiomyocyte proliferation and cardiac regeneration

Yi Xiao, Hao Zhang, Xu Liu, Pengfei Xu, Heng Du, Jiawan Wang, Jianghua Shen, Yujing Li, Yuhan Wang, Chuting He, Haiping Feng, Jingfang Liu, Yanan Zhou, Siqi Liu, Zeyu Gao, Jingyi Zang, Jinmiao Bi, Tie-Shan Tang, Qi Gu, Tuo Wei, Jun Wang and Moshi Song ()
Additional contact information
Yi Xiao: Chinese Academy of Sciences
Hao Zhang: Chinese Academy of Sciences
Xu Liu: Chinese Academy of Sciences
Pengfei Xu: Chinese Academy of Sciences
Heng Du: Chinese Academy of Sciences
Jiawan Wang: Department of Anesthesiology
Jianghua Shen: Chinese Academy of Sciences
Yujing Li: Chinese Academy of Sciences
Yuhan Wang: Chinese Academy of Sciences
Chuting He: Chinese Academy of Sciences
Haiping Feng: Chinese Academy of Sciences
Jingfang Liu: Chinese Academy of Sciences
Yanan Zhou: Chinese Academy of Sciences
Siqi Liu: Chinese Academy of Sciences
Zeyu Gao: Chinese Academy of Sciences
Jingyi Zang: Chinese Academy of Sciences
Jinmiao Bi: Chinese Academy of Sciences
Tie-Shan Tang: Chinese Academy of Sciences
Qi Gu: Chinese Academy of Sciences
Tuo Wei: Chinese Academy of Sciences
Jun Wang: University of Chinese Academy of Sciences
Moshi Song: Chinese Academy of Sciences

Nature Communications, 2025, vol. 16, issue 1, 1-20

Abstract: Abstract Heart injury has been characterized by the irreversible loss of cardiomyocytes comprising the contractile tissues of the heart and thus strategies enabling adult cardiomyocyte proliferation are highly desired for treating various heart diseases. Here, we test the ability of human induced pluripotent stem cell-derived primitive macrophages (hiPMs) and their conditioned medium (hiPM-cm) to promote human cardiomyocyte proliferation and enhance cardiac regeneration in adult mice. We find that hiPMs promote human cardiomyocyte proliferation, which is recapitulated by hiPM-cm through the activation of multiple pro-proliferative pathways, and a secreted proteome analysis identifies five proteins participating in this activation. Subsequent in vivo experiments show that hiPM-cm promotes adult cardiomyocyte proliferation in mice. Lastly, hiPM-cm enhances cardiac regeneration and improves contractile function in injured adult mouse hearts. Together, our study demonstrates the efficacy of using hiPM-cm in promoting adult cardiomyocyte proliferation and cardiac regeneration to serve as an innovative treatment for heart disease.

Date: 2025
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-025-58301-8 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-58301-8

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-025-58301-8

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().