Microecology in vitro model replicates the human skin microbiome interactions

Pan Wang, Huijuan Li, Xingjiang Zhang, Xiaoxun Wang, Wenwen Sun, Xiaoya Zhang, Baiyi Chi, Yuyo Go, Xi Hui Felicia Chan, Jianxin Wu () and Qing Huang ()
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Pan Wang: China Pharmaceutical University
Huijuan Li: China Pharmaceutical University
Xingjiang Zhang: China Pharmaceutical University
Xiaoxun Wang: China Pharmaceutical University
Wenwen Sun: China Pharmaceutical University
Xiaoya Zhang: China Pharmaceutical University
Baiyi Chi: China Pharmaceutical University
Yuyo Go: Parks Road
Xi Hui Felicia Chan: 183 Pembroke Street
Jianxin Wu: China Pharmaceutical University
Qing Huang: China Pharmaceutical University

Nature Communications, 2025, vol. 16, issue 1, 1-14

Abstract: Abstract Skin microecology involves a dynamic equilibrium among the host, microbiome, and internal/external environments. This equilibrium, shaped by multifactorial interactions, reflects individual specificity and diversity. Creating a replicable in vitro skin microecological model is highly challenging. Here, we introduce a mimicked stratum corneum microecology model (SCmic). It uses light cured crosslinked hydrogels as a scaffold and moisture source, and nonviable epidermal cells as the main nutrient. This setup establishes a suitable, stable, and reproducible microecology for microbiome colonization. Notably, it replicates the normal/oily skin microbiota with no significant differences from the original native microbiota at the genus level. Simultaneously, we have developed a standardized human skin microbiota model (Hcm), featuring seven dominant strains that form a representative microbial community. The models provide highly convenient approaches for exploring the intricate mutual interactions among skin microecology, influence of microbiota on skin health, and metabolism of chemical substances by microbiota.

Date: 2025
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DOI: 10.1038/s41467-025-58377-2

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