Autophagy controls differentiation of Drosophila blood cells by regulating Notch levels in response to nutrient availability

Maximiliano J. Katz, Felipe Rodríguez, Fermín Evangelisti, Agustina G. Borrat, Sebastián Perez-Pandolfo, Tomás Peters, Natalia Sommario, Graciela L. Boccaccio, Mariana Melani and Pablo Wappner ()
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Maximiliano J. Katz: Universidad de Buenos Aires. Paraguay 2155
Felipe Rodríguez: Instituto Leloir
Fermín Evangelisti: Instituto Leloir
Agustina G. Borrat: Instituto Leloir
Sebastián Perez-Pandolfo: Instituto Leloir
Tomás Peters: Instituto Leloir
Natalia Sommario: Instituto Leloir
Graciela L. Boccaccio: Instituto Leloir
Mariana Melani: Instituto Leloir
Pablo Wappner: Instituto Leloir

Nature Communications, 2025, vol. 16, issue 1, 1-17

Abstract: Abstract Drosophila larval hematopoiesis takes place at the lymph gland, where blood cell progenitors differentiate into two possible cell types: Plasmatocytes, analogous to mammalian macrophages, or crystal cells that share features with mammalian megakaryocytes; a third cell type, the lamellocytes, develop only upon specific immune challenges. Here we show that autophagy inhibition in blood cell progenitors results in augmented crystal cell differentiation due to Notch accumulation. Notch activation during hematopoiesis depends on the endocytic pathway, which crosstalks with autophagy: While Notch activation depends on endocytosis and endosomal maturation, Notch lysosomal degradation requires autophagy. TOR signaling inhibits autophagosome biogenesis that in turn prevents the formation of Notch-containing amphisomes, which are necessary for Notch lysosomal destruction. Reduction of Notch lysosomal degradation shifts the balance towards Notch activation at endosomal membranes, thereby enhancing differentiation of crystal cells. Our work therefore defines a mechanism of regulation of immune cell differentiation in response to the nutritional status of the organism.

Date: 2025
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DOI: 10.1038/s41467-025-58389-y

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