Granulosa cell transcription is similarly impacted by superovulation and aging and predicts early embryonic trajectories

Klaudija Daugelaite, Perrine Lacour, Ivana Winkler, Marie-Luise Koch, Anja Schneider, Nina Schneider, Francesca Coraggio, Alexander Tolkachov, Xuan Phuoc Nguyen, Adriana Vilkaite, Julia Rehnitz, Duncan T. Odom () and Angela Goncalves ()
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Klaudija Daugelaite: German Cancer Research Center (DKFZ)
Perrine Lacour: Ruprecht-Karl-University Heidelberg
Ivana Winkler: German Cancer Research Center (DKFZ)
Marie-Luise Koch: German Cancer Research Center (DKFZ)
Anja Schneider: German Cancer Research Center (DKFZ)
Nina Schneider: German Cancer Research Center (DKFZ)
Francesca Coraggio: German Cancer Research Center (DKFZ)
Alexander Tolkachov: German Cancer Research Center (DKFZ)
Xuan Phuoc Nguyen: University Women’s Hospital Heidelberg
Adriana Vilkaite: University Women’s Hospital Heidelberg
Julia Rehnitz: University Women’s Hospital Heidelberg
Duncan T. Odom: German Cancer Research Center (DKFZ)
Angela Goncalves: German Cancer Research Center (DKFZ)

Nature Communications, 2025, vol. 16, issue 1, 1-17

Abstract: Abstract In vitro fertilization efficiency is limited in part because a fraction of retrieved oocytes fails to fertilize. Accurately evaluating their quality could significantly improve in vitro fertilization efficiency, which would require better understanding how their maturation may be disrupted. Here, we quantitatively investigate the interplay between superovulation and aging in mouse oocytes and their paired granulosa cells using a newly adapted experimental methodology. We test the hypothesis that superovulation disrupts oocyte maturation, revealing the key intercellular communication pathways dysregulated at the transcriptional level by forced hormonal stimulation. We further demonstrate that granulosa cell transcriptional markers can prospectively predict an associated oocyte’s early developmental potential. By using naturally ovulated old mice as a non-stimulated reference, we show that aging and superovulation dysregulate similar genes and interact with each other. By comparing mice and human transcriptional responses of granulosa cells, we find that age-related dysregulation of hormonal responses and cell cycle pathways are shared, though substantial divergence exists in other pathways.

Date: 2025
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DOI: 10.1038/s41467-025-58451-9

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