The essential calcium channel of sperm CatSper is temperature-gated

Dilip K. Swain, Citlalli Vergara, Júlia Castro-Arnau and Polina V. Lishko ()
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Dilip K. Swain: School of Medicine
Citlalli Vergara: School of Medicine
Júlia Castro-Arnau: School of Medicine
Polina V. Lishko: School of Medicine

Nature Communications, 2025, vol. 16, issue 1, 1-14

Abstract: Abstract The flagellar calcium channel CatSper is essential for male fertility, as it regulates calcium influx to trigger the hyperactive motility required for sperm to fertilize the egg. Precise activation of CatSper is critical, as premature activation can impair sperm function. While optimal temperature is known to influence fertilization, its effect on CatSper remains unknown. By directly recording from mouse spermatozoa, we reveal that CatSper functions as a temperature-gated ion channel, with a thermal threshold of 33.5 °C and a temperature coefficient Q10 of 5.1. Additionally, we show that physiological levels of spermine reversibly inhibit CatSper’s temperature gating, protecting against premature activation. Our findings highlight for the first time the presence of the temperature-gating modality of CatSper and reveal the protective role of spermine, a major component of seminal plasma. These results emphasize the need to maintain testes below 34 °C for optimal fertility and advance understanding of CatSper regulation in male fertility.

Date: 2025
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DOI: 10.1038/s41467-025-58824-0

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