Auto-sumoylation of the yeast Ubc9 E2 SUMO-conjugating enzyme extends cellular lifespan

Dong-Won Jeong, Do Yoon Lee, Seung Yeon Kim, Seok-Won Jeoung, Dejian Zhao, James Knight, TuKiet T. Lam, Jong Hwa Jin, Hyun-Shik Lee, Mark Hochstrasser () and Hong-Yeoul Ryu ()
Additional contact information
Dong-Won Jeong: Kyungpook National University
Do Yoon Lee: Kyungpook National University
Seung Yeon Kim: Kyungpook National University
Seok-Won Jeoung: Kyungpook National University
Dejian Zhao: Yale University
James Knight: Yale University
TuKiet T. Lam: Yale School of Medicine
Jong Hwa Jin: New Drug Development Center
Hyun-Shik Lee: Kyungpook National University
Mark Hochstrasser: Yale University
Hong-Yeoul Ryu: Kyungpook National University

Nature Communications, 2025, vol. 16, issue 1, 1-17

Abstract: Abstract Calorie restriction (CR) provides anti-aging benefits through diverse processes, such as reduced metabolism and growth and increased mitochondrial activity. Although controversy still exists regarding CR-mediated lifespan effects, many researchers are seeking interventions that mimic the effects of CR. Yeast has proven to be a useful model system for aging studies, including CR effects. We report here that yeast adapted through in vitro evolution to the severe cellular stress caused by loss of the Ulp2 SUMO-specific protease exhibit both enhanced growth rates and replicative lifespan, and they have altered gene expression profiles similar to those observed in CR. Notably, in certain evolved ulp2Δ lines, an increase in the auto-sumoylation of Ubc9 E2 SUMO-conjugating enzyme results in altered regulation of multiple targets involved in energy metabolism and translation at both transcriptional and post-translational levels. This increase is essential for the survival of aged cells and CR-mediated lifespan extension. Thus, we suggest that high Ubc9 auto-sumoylation exerts potent anti-aging effects by promoting efficient energy metabolism-driven improvements in cell replication abilities. This potential could be therapeutically explored for the development of promising CR-mimetic strategies.

Date: 2025
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-025-58925-w Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-58925-w

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-025-58925-w

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().