C. elegans SSNA-1 is required for the structural integrity of centrioles and bipolar spindle assembly

Jason A. Pfister, Lorenzo Agostini, Lorène Bournonville, Aurélien Perrier, Prabhu Sankaralingam, Zachary G. Bell, Virginie Hamel, Paul Guichard, Christian Biertümpfel, Naoko Mizuno () and Kevin F. O’Connell ()
Additional contact information
Jason A. Pfister: National Institutes of Health
Lorenzo Agostini: National Institutes of Health
Lorène Bournonville: University of Geneva
Aurélien Perrier: University of Geneva
Prabhu Sankaralingam: National Institutes of Health
Zachary G. Bell: National Institutes of Health
Virginie Hamel: University of Geneva
Paul Guichard: University of Geneva
Christian Biertümpfel: National Institutes of Health
Naoko Mizuno: National Institutes of Health
Kevin F. O’Connell: National Institutes of Health

Nature Communications, 2025, vol. 16, issue 1, 1-16

Abstract: Abstract Centrioles play key roles in mitotic spindle assembly. Once assembled, centrioles exhibit long-term stability, but how stability is achieved and how it is regulated are not completely understood. In this study we show that SSNA-1, the Caenorhabditis elegans ortholog of Sjogren’s Syndrome Nuclear Antigen 1, is a constituent of centrioles and centriole satellite-like structures. A deletion of ssna-1 results in the formation of extra centrioles. We show that SSNA-1 genetically interacts with the centriole stability factor SAS-1 and is required post assembly for centriole structural integrity. In SSNA-1’s absence, centrioles assemble but fracture leading to extra spindle poles. However, if the efficiency of cartwheel assembly is reduced, the absence of SSNA-1 results in daughter centriole loss and monopolar spindles, indicating that the cartwheel and SSNA-1 cooperate to stabilize centrioles during assembly. Our work thus shows that SSNA-1 contributes to centriole stability during and after assembly, thereby ensuring proper centriole number.

Date: 2025
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-025-59939-0 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-59939-0

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-025-59939-0

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().