Spatiotemporal development of expanding bacterial colonies driven by emergent mechanical constraints and nutrient gradients

Harish Kannan, Hui Sun (), Mya Warren, Tolga Çağlar, Pantong Yao, Brian R. Taylor, Kinshuk Sahu, Daotong Ge, Matteo Mori, David Kleinfeld, JiaJia Dong (), Bo Li () and Terence Hwa ()
Additional contact information
Harish Kannan: San Diego
Hui Sun: California State University
Mya Warren: San Diego
Tolga Çağlar: San Diego
Pantong Yao: San Diego
Brian R. Taylor: San Diego
Kinshuk Sahu: San Diego
Daotong Ge: San Diego
Matteo Mori: San Diego
David Kleinfeld: San Diego
JiaJia Dong: Bucknell University
Bo Li: San Diego
Terence Hwa: San Diego

Nature Communications, 2025, vol. 16, issue 1, 1-17

Abstract: Abstract Bacterial colonies growing on solid surfaces can exhibit robust expansion kinetics, with constant radial growth and saturating vertical expansion, suggesting a common developmental program. Here, we study this process for Escherichia coli cells using a combination of modeling and experiments. We show that linear radial colony expansion is set by the verticalization of interior cells due to mechanical constraints rather than radial nutrient gradients as commonly assumed. In contrast, vertical expansion slows down from an initial linear regime even while radial expansion continues linearly. This vertical slowdown is due to limitation of cell growth caused by vertical nutrient gradients, exacerbated by concurrent oxygen depletion. Starvation in the colony interior results in a distinct death zone which sets in as vertical expansion slows down, with the death zone increasing in size along with the expanding colony. Thus, our study reveals complex heterogeneity within simple monoclonal bacterial colonies, especially along the vertical dimension. The intricate dynamics of such emergent behavior can be understood quantitatively from an interplay of mechanical constraints and nutrient gradients arising from obligatory metabolic processes.

Date: 2025
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DOI: 10.1038/s41467-025-60004-z

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