A searchable atlas of pathogen-sensitive lncRNA networks in human macrophages
Nils Schmerer,
Harshavardhan Janga,
Michelle Aillaud,
Janina Hoffmann,
Marina Aznaourova,
Sarah Wende,
Henrike Steding,
Luke D. Halder,
Michael Uhl,
Fabian Boldt,
Thorsten Stiewe,
Andrea Nist,
Lukas Jerrentrup,
Andreas Kirschbaum,
Clemens Ruppert,
Oliver Rossbach,
Evgenia Ntini,
Annalisa Marsico,
Chanil Valasarajan,
Rolf Backofen,
Uwe Linne,
Soni S. Pullamsetti,
Bernd Schmeck and
Leon N. Schulte ()
Additional contact information
Nils Schmerer: Philipps University Marburg
Harshavardhan Janga: Philipps University Marburg
Michelle Aillaud: Philipps University Marburg
Janina Hoffmann: Philipps University Marburg
Marina Aznaourova: Philipps University Marburg
Sarah Wende: Philipps University Marburg
Henrike Steding: Philipps University Marburg
Luke D. Halder: Philipps University Marburg
Michael Uhl: University of Freiburg
Fabian Boldt: Philipps University Marburg
Thorsten Stiewe: German Center for Lung Research (DZL)
Andrea Nist: University of Marburg
Lukas Jerrentrup: Philipps University Marburg
Andreas Kirschbaum: University Hospital Giessen and Marburg (UKGM)
Clemens Ruppert: German Center for Lung Research (DZL)
Oliver Rossbach: Justus Liebig University Giessen
Evgenia Ntini: Max Planck Institute for Molecular Genetics
Annalisa Marsico: Max Planck Institute for Molecular Genetics
Chanil Valasarajan: Universities of Giessen and Marburg Lung Center (UGMLC)
Rolf Backofen: University of Freiburg
Uwe Linne: Philipps University
Soni S. Pullamsetti: German Center for Lung Research (DZL)
Bernd Schmeck: Philipps University Marburg
Leon N. Schulte: Philipps University Marburg
Nature Communications, 2025, vol. 16, issue 1, 1-17
Abstract:
Abstract Long noncoding RNAs (lncRNA) are crucial yet underexplored regulators of human immunity. Here we develop GRADR, a method integrating gradient profiling with RNA-binding proteome analysis, to map the protein interactomes of all expressed RNAs in a single experiment to study mechanisms of lncRNA-mediated regulation of human primary macrophages. Applying GRADR alongside CRISPR-multiomics, we reveal a network of NFκB-dependent lncRNAs, including LINC01215, AC022816.1 and ROCKI, which modulate distinct aspects of macrophage immunity, particularly through interactions with mRNA-processing factors, such as hnRNP proteins. We further uncover the function of ROCKI in repressing the messenger of the anti-inflammatory GATA2 transcription factor, thus promoting macrophage activation. Lastly, all data are consolidated in the SMyLR web interface, a searchable reference catalog for exploring lncRNA functions and pathway-dependencies in immune cells. Our results thus not only highlight the important functions of lncRNAs in immune regulation, but also provide a rich resource for lncRNA studies.
Date: 2025
References: Add references at CitEc
Citations:
Downloads: (external link)
https://www.nature.com/articles/s41467-025-60084-x Abstract (text/html)
Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.
Export reference: BibTeX
RIS (EndNote, ProCite, RefMan)
HTML/Text
Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-60084-x
Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/
DOI: 10.1038/s41467-025-60084-x
Access Statistics for this article
Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie
More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().