Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma

Lakkhana Sadaow, Rutchanee Rodpai, Michael J. Smout, Rie Nakajima, Patcharaporn Boonroumkaew, Javier Sotillo, Bemnet A. Tedla, Vor Luvira, Amnat Kitkhuandee, Krisada Paonariang, Wattana Sukeepaisarnjaroen, Hiroshi Yamasaki, Sutas Suttiprapa, Thewarach Laha, Banchob Sripa, Rafael Assis, Aarti Jain, Wannaporn Ittiprasert, Victoria H. Mann, Yide Wong, Philip L. Felgner, Wanchai Maleewong, Paul J. Brindley (), Alex Loukas () and Pewpan M. Intapan
Additional contact information
Lakkhana Sadaow: Khon Kaen University
Rutchanee Rodpai: Khon Kaen University
Michael J. Smout: James Cook University
Rie Nakajima: University of California Irvine
Patcharaporn Boonroumkaew: Khon Kaen University
Javier Sotillo: Instituto de Salud Carlos III
Bemnet A. Tedla: James Cook University
Vor Luvira: Khon Kaen University
Amnat Kitkhuandee: Khon Kaen University
Krisada Paonariang: Khon Kaen University
Wattana Sukeepaisarnjaroen: Khon Kaen University
Hiroshi Yamasaki: National Institute of Infectious Diseases
Sutas Suttiprapa: Khon Kaen University
Thewarach Laha: Khon Kaen University
Banchob Sripa: Khon Kaen University
Rafael Assis: University of California Irvine
Aarti Jain: University of California Irvine
Wannaporn Ittiprasert: George Washington University
Victoria H. Mann: George Washington University
Yide Wong: James Cook University
Philip L. Felgner: University of California Irvine
Wanchai Maleewong: Khon Kaen University
Paul J. Brindley: George Washington University
Alex Loukas: James Cook University
Pewpan M. Intapan: Khon Kaen University

Nature Communications, 2025, vol. 16, issue 1, 1-12

Abstract: Abstract Sensitive diagnostics are needed to improve management and surveillance of opisthorchiasis and opisthorchiasis-associated cholangiocarcinoma (CCA) throughout East Asia. Herein we generate and screen an Opisthorchis viverrini recombinant secreted proteome to identity antibody biomarkers of liver fluke infection and CCA with sera from study participants in endemic populations and evaluate their utility as point-of-care immunochromatographic tests (PoC-ICTs). We incorporate two of the most promising antigens from the proteome array screen, P1 and P9, into PoC-ICTs to further validate their diagnostic performance. The P9-IgG4 PoC-ICT is superior amongst the single recombinant antigen tests for diagnosing fluke infection as well as fluke-induced CCA, and out-performs parasite crude extract-IgG ICTs. Here we identify two biomarkers of O. viverrini infection and infection-associated CCA that could form the basis of novel antibody serodiagnostic tests for human liver fluke infection and associated cancer.

Date: 2025
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DOI: 10.1038/s41467-025-61043-2

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