Transcriptomics and trans-organellar complementation reveal limited signaling of 12-cis-oxo-phytodienoic acid during early wound response in Arabidopsis

Khansa Mekkaoui, Ranjit Baral, Fiona Smith, Moritz Klein, Ivo Feussner and Bettina Hause ()
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Khansa Mekkaoui: Weinberg 3
Ranjit Baral: Weinberg 3
Fiona Smith: Weinberg 3
Moritz Klein: Justus-von-Liebig-Weg 11
Ivo Feussner: Justus-von-Liebig-Weg 11
Bettina Hause: Weinberg 3

Nature Communications, 2025, vol. 16, issue 1, 1-17

Abstract: Abstract 12-cis-oxo-phytodienoic acid (OPDA), a precursor of jasmonoyl-isoleucine (JA-Ile), is known to have distinct signaling roles in Arabidopsis, as shown in studies using the opr3 mutant, which lacks OPDA REDUCTASE3 (OPR3). This mutant, however, accumulates low levels of JA-Ile through an OPR2-mediated bypass. To investigate OPDA signaling, the wound-induced transcriptome of the opr2opr3 mutant is compared to that of wild-type and allene oxide synthase mutant. Endogenous OPDA shows no unique transcriptional signature under control or wounding conditions, and previously identified OPDA-responsive genes are wound-induced independently of OPDA. Applying OPDA to opr2opr3 triggers a distinct response suggesting compartmentalization of endogenously formed OPDA. Trans-organellar complementation reveals that expression of OPR3 or OPR2 in opr2opr3 restores JA-Ile production regardless of localization, whereas mitochondrial targeted OPR1 exhibiting low OPDA/4,5-ddh-JA conversion activity does not. Our findings show that OPDA primarily serves as a JA precursor with limited independent signaling functions in the early wound response.

Date: 2025
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DOI: 10.1038/s41467-025-61832-9

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