Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli

Yannick Baffert, Nathan Fraikin, Yasmine Makhloufi, Julie Baltenneck, Marie-Eve Val, Annick Dedieu-Berne, Jonathan Degosserie, Bogdan I. Iorga, Pierre Bogaerts, Erwan Gueguen, Christian Lesterlin and Sarah Bigot ()
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Yannick Baffert: UMR5086
Nathan Fraikin: UMR5086
Yasmine Makhloufi: UMR5086
Julie Baltenneck: UMR 5240
Marie-Eve Val: Département Génomes et Génétique
Annick Dedieu-Berne: UMR5086
Jonathan Degosserie: CHU UCL Namur
Bogdan I. Iorga: Institut de Chimie des Substances Naturelles
Pierre Bogaerts: CHU UCL Namur
Erwan Gueguen: UMR 5240
Christian Lesterlin: UMR5086
Sarah Bigot: UMR5086

Nature Communications, 2025, vol. 16, issue 1, 1-13

Abstract: Abstract Conjugative plasmids are the main drivers of antibiotic resistance dissemination contributing to the emergence and extensive spread of multidrug resistance clinical bacterial pathogens. pOXA-48 plasmids, belonging to the IncL group, emerge as the primary vehicle for carbapenem resistance in Enterobacteriaceae. Despite the problematic prevalence of pOXA-48, most research focus on epidemiology and genomics, leaving gaps in our understanding of the mechanisms behind its propagation. In this study, we use a transposon sequencing approach to identify genetic elements critical for plasmid stability, replication, and conjugative transfer. Our results identify a novel type I toxin-antitoxin system, uncharacterized essential maintenance factors, and components of the type IV secretion system and regulatory elements crucial for conjugation. This study advances our understanding of pOXA-48 biology, providing key insights into the genetic factors underlying its successful maintenance and spread in bacterial populations.

Date: 2025
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DOI: 10.1038/s41467-025-62404-7

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