Opposing regulation of the K63-linked polyubiquitination of RIPK3 by SMURF1 and USP5 in necroptosis

Chi Hyun Hwang, Minhong Lee, Ju Won Kim, Young Woo Nam, Gyuho Hwang, Hyun Sung Ryu, Jinho Seo, Eun-Woo Lee, Hyuk Wan Ko and Jaewhan Song ()
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Chi Hyun Hwang: Yonsei University
Minhong Lee: Yonsei University
Ju Won Kim: Yonsei University
Young Woo Nam: Yonsei University
Gyuho Hwang: Yonsei University
Hyun Sung Ryu: Yonsei University
Jinho Seo: Korea Research Institute of Bioscience and Biotechnology (KRIBB)
Eun-Woo Lee: KRIBB
Hyuk Wan Ko: Yonsei University
Jaewhan Song: Yonsei University

Nature Communications, 2025, vol. 16, issue 1, 1-16

Abstract: Abstract Receptor-interacting protein kinase 3 (RIPK3), a key regulator of necroptosis, is modulated by ubiquitination through various E3 ligases and deubiquitinases. However, the effects of different polyubiquitination processes on RIPK3 and necroptosis remain unclear. Using a proteomic approach, we identify SMAD Ubiquitination Regulatory Factor 1 (SMURF1) and Ubiquitin-specific peptidase 5 (USP5) as crucial regulators of RIPK3 within the necrosome during necroptosis. SMURF1 facilitates K63 polyubiquitination of RIPK3 at lysine 55 and 363, inhibiting necrosome formation and necroptosis. SMURF1 depletion accelerates necroptosis, while the reintroduction of functional SMURF1 reverses this. Conversely, USP5 acts as a deubiquitinase, removing K63 ubiquitin chains and promoting necroptosis. Reducing SMURF1, using a RIPK3 mutant defective in SMURF1-mediated ubiquitination, or overexpressing USP5 enhances necroptosis in leukaemia cells, leading to reduced tumour growth in xenograft models treated with birinapant and emricasan. These findings highlight the opposing regulation of K63-linked polyubiquitination of RIPK3 by SMURF1 and USP5 in necroptosis.

Date: 2025
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DOI: 10.1038/s41467-025-62723-9

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