Cryo-EM identifies F-ENA of Bacillus thuringiensis as a widespread family of endospore appendages across Firmicutes

Mike Sleutel (), Adrià Sogues, Nani Gerven, Unni Lise Jonsmoen, Inge Molle, Marcus Fislage, Laurent Dirk Theunissen, Nathan F. Bellis, Diana P. Baquero, Edward H. Egelman, Mart Krupovic, Fengbin Wang, Marina Aspholm and Han Remaut ()
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Mike Sleutel: Vrije Universiteit Brussel
Adrià Sogues: Vrije Universiteit Brussel
Nani Gerven: Vrije Universiteit Brussel
Unni Lise Jonsmoen: Norwegian University of Life Sciences (NMBU)
Inge Molle: Vrije Universiteit Brussel
Marcus Fislage: Vrije Universiteit Brussel
Laurent Dirk Theunissen: Vrije Universiteit Brussel
Nathan F. Bellis: University of Alabama at Birmingham
Diana P. Baquero: Cell Biology and Virology of Archaea Unit
Edward H. Egelman: University of Virginia School of Medicine
Mart Krupovic: Cell Biology and Virology of Archaea Unit
Fengbin Wang: University of Alabama at Birmingham
Marina Aspholm: Norwegian University of Life Sciences (NMBU)
Han Remaut: Vrije Universiteit Brussel

Nature Communications, 2025, vol. 16, issue 1, 1-15

Abstract: Abstract For over 100 years, Bacillus thuringiensis (Bt) has been used as an agricultural biopesticide to control pests caused by insect species in the orders of Lepidoptera, Diptera, and Coleoptera. Under nutrient starvation, Bt cells differentiate into spores and associated toxin crystals that can adopt biofilm-like aggregates. We reveal that such Bt spore/toxin biofilms are embedded in a fibrous extrasporal matrix, and using cryoID, we resolved the structure and molecular identity of an uncharacterized type of pili, referred to here as Fibrillar ENdospore Appendages or F-ENA. F-ENA are monomolecular protein filaments anchored to the exosporium and tipped with a flexible fibrillum. Phylogenetic and structural analyses reveal that F-ENA are conserved in Bacilli and Clostridia, featuring head-neck domains with β-barrel necks that interlock via N-terminal hook peptides. In Bacillus, two collagen-like proteins (F-Anchor and F-BclA), respectively, tether F-ENA and form the distal tip. Sedimentation assays suggest F-ENA promotes spore clustering via F-BclA contacts and/or filament bundling.

Date: 2025
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DOI: 10.1038/s41467-025-62896-3

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