IWS1 positions downstream DNA to globally stimulate Pol II elongation

Aiturgan Zheenbekova, James L. Walshe, Moritz Ochmann, Moritz Bäuerle, Ute Neef, Kerstin C. Maier, Petra Rus, Yumeng Yan, Henning Urlaub, Patrick Cramer () and Kristina Žumer ()
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Aiturgan Zheenbekova: Max Planck Institute for Multidisciplinary Sciences
James L. Walshe: Max Planck Institute for Multidisciplinary Sciences
Moritz Ochmann: Max Planck Institute for Multidisciplinary Sciences
Moritz Bäuerle: Max Planck Institute for Multidisciplinary Sciences
Ute Neef: Max Planck Institute for Multidisciplinary Sciences
Kerstin C. Maier: Max Planck Institute for Multidisciplinary Sciences
Petra Rus: Max Planck Institute for Multidisciplinary Sciences
Yumeng Yan: Max Planck Institute for Multidisciplinary Sciences
Henning Urlaub: Max Planck Institute for Multidisciplinary Sciences
Patrick Cramer: Max Planck Institute for Multidisciplinary Sciences
Kristina Žumer: Max Planck Institute for Multidisciplinary Sciences

Nature Communications, 2025, vol. 16, issue 1, 1-15

Abstract: Abstract The protein IWS1 (Interacts with SPT6 1) is implicated in transcription-associated processes, but a direct role in RNA polymerase (Pol) II function is unknown. Here, we use multi-omics kinetic analysis after rapid depletion of IWS1 in human cells to show that loss of IWS1 results in a global decrease of RNA synthesis and a global reduction in Pol II elongation velocity. We then resolve the cryo-EM structure of the activated Pol II elongation complex with bound IWS1 and elongation factor ELOF1 and show that IWS1 acts as a scaffold and positions downstream DNA within the cleft of Pol II. In vitro assays show that the disordered C-terminal region of IWS1 that contacts the cleft of Pol II is responsible for stimulation of Pol II activity and is aided by ELOF1. Finally, we find that the defect in transcription upon IWS1 depletion leads to a decrease of histone H3 tri-methylation at residue lysine-36 (H3K36me3), but that this secondary effect is an indirect function of IWS1. In summary, our structure-function analysis establishes IWS1 as a Pol II-associated elongation factor that acts globally to stimulate Pol II elongation velocity and ensure proper co-transcriptional histone methylation.

Date: 2025
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DOI: 10.1038/s41467-025-62913-5

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