Real-time, high-throughput super-resolution microscopy via panoramic integration

Kyungduck Yoon, Hansol Yoon, Kidan Tadesse, Zhi Ling, Biagio Mandracchia, Sayantan Datta, G. Ozan Bozdag, Anthony J. Burnetti, William C. Ratcliff and Shu Jia ()
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Kyungduck Yoon: Georgia Institute of Technology
Hansol Yoon: Georgia Institute of Technology
Kidan Tadesse: Georgia Institute of Technology
Zhi Ling: Georgia Institute of Technology
Biagio Mandracchia: Georgia Institute of Technology
Sayantan Datta: Georgia Institute of Technology
G. Ozan Bozdag: Georgia Institute of Technology
Anthony J. Burnetti: Georgia Institute of Technology
William C. Ratcliff: Georgia Institute of Technology
Shu Jia: Georgia Institute of Technology

Nature Communications, 2025, vol. 16, issue 1, 1-9

Abstract: Abstract We introduce super-resolution panoramic integration (SPI), an on-the-fly microscopy technique enabling instantaneous generation of subdiffractional images concurrently with scalable, high-throughput screening. SPI leverages multifocal optical rescaling, high-content sweeping, and synchronized line-scan readout while preserving minimal post-processing and compatibility with epi-fluorescence settings. We demonstrate SPI for various subcellular and populational morphology, function, and heterogeneity. This versatile platform offers a practical pathway toward biological insights beyond traditional optical and computational constraints.

Date: 2025
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DOI: 10.1038/s41467-025-64368-0

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