Stress changes the material state of a bacterial biomolecular condensate and shifts its function from mRNA decay to storage

Luis A. Ortiz-Rodríguez, Hadi Yassine, Ali Hatami, Vidhyadhar Nandana, Christopher A. Azaldegui, Jiayu Cheng, Yingxi Zhu, Jared M. Schrader () and Julie S. Biteen ()
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Luis A. Ortiz-Rodríguez: University of Michigan
Hadi Yassine: Wayne State University
Ali Hatami: Wayne State University
Vidhyadhar Nandana: Wayne State University
Christopher A. Azaldegui: University of Michigan
Jiayu Cheng: University of Michigan
Yingxi Zhu: Wayne State University
Jared M. Schrader: Wayne State University
Julie S. Biteen: University of Michigan

Nature Communications, 2025, vol. 16, issue 1, 1-19

Abstract: Abstract Bacterial ribonucleoprotein bodies (BR-bodies) are dynamic biomolecular condensates that play a pivotal role in RNA metabolism. We investigated how BR-bodies significantly influence mRNA fate by transitioning between liquid- and solid-like states in response to stress. With a combination of single-molecule and bulk fluorescence microscopy, biochemical assays, and quantitative analyses, we determine that BR-bodies promote efficient mRNA decay in a liquid-like condensate during exponential growth. On the other hand, BR-bodies are repurposed from sites of mRNA decay to reservoirs for mRNA storage under stress; a functional change that is enabled by their transition to a more rigid state, marked by reduced internal dynamics, increased molecular density, and prolonged residence time of Ribonuclease E. Furthermore, we manipulated ATP levels and translation rates, and we conclude that the accumulation of ribosome-depleted mRNA is a key factor driving BR-body rigification, and that condensate maturation further contributes to this process. Upon nutrient replenishment, stationary-phase BR-bodies disassemble, releasing stored mRNAs for rapid translation, demonstrating that BR-body function is governed by a reversible mechanism for resource management. These findings reveal adaptive strategies by which bacteria regulate RNA metabolism through condensate-mediated control of mRNA decay and storage.

Date: 2025
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DOI: 10.1038/s41467-025-65358-y

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