Iron deficiency changes regulatory mechanisms governing sieve element cell differentiation

Imani Madison, Eli D. Buckner, Maria Angels de Luis Balaguer, Jina Song, Dipali Srivastava, Devarshi Selote, Aitch Hunt, Eduardo Bueso, Rosangela Sozzani, Cranos Williams and Terri A. Long ()
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Imani Madison: North Carolina State University, Department of Plant and Microbial Biology
Eli D. Buckner: North Carolina State University, Department of Electrical and Computer Engineering
Maria Angels de Luis Balaguer: North Carolina State University, Department of Plant and Microbial Biology
Jina Song: North Carolina State University, Department of Plant and Microbial Biology
Dipali Srivastava: North Carolina State University, Department of Plant and Microbial Biology
Devarshi Selote: North Carolina State University, Department of Plant and Microbial Biology
Aitch Hunt: North Carolina State University, Department of Plant and Microbial Biology
Eduardo Bueso: Camino de Vera, Instituto de Biología Molecular y Celular de Plantas, Universitat Politècnica de València-Consejo Superior de Investigaciones Científicas
Rosangela Sozzani: North Carolina State University, Department of Plant and Microbial Biology
Cranos Williams: North Carolina State University, Department of Electrical and Computer Engineering
Terri A. Long: North Carolina State University, Department of Plant and Microbial Biology

Nature Communications, 2025, vol. 16, issue 1, 1-14

Abstract: Abstract Plant cell differentiation incorporates environmental cues over time to optimize overall growth. Iron deficiency influences development, such as root hair, cortical, and endodermal cell differentiation. However, the mechanisms by which iron deficiency regulates cell differentiation are not well characterized. Root sieve elements serve as an excellent model for cell differentiation since all cells, from undifferentiated to differentiated, are present in a distinct cell file. Here, we use semi-automated image analysis to show that iron deficiency delays sieve element differentiation, particularly enucleation and cell wall thickening, and consequently delays phloem sap unloading to roots. Using Dynamic Bayesian modeling we also characterize how iron deficiency changes the fundamental structure of the gene regulatory network associated with sieve element differentiation. We identify DOF1.5 as a positive regulator of sieve element enucleation and, consequently, of root sap translocation. These results clarify how abiotic stress can influence overall plant growth as a consequence of negatively influencing vascular differentiation.

Date: 2025
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DOI: 10.1038/s41467-025-65428-1

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