A plasmid-based multigene expression system for mammalian cells

Andrijana Kriz, Katharina Schmid, Nadia Baumgartner, Urs Ziegler, Imre Berger, Kurt Ballmer-Hofer and Philipp Berger ()
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Andrijana Kriz: Paul Scherrer Institute, Biomolecular Research, Molecular Cell Biology
Katharina Schmid: Paul Scherrer Institute, Biomolecular Research, Molecular Cell Biology
Nadia Baumgartner: Paul Scherrer Institute, Biomolecular Research, Molecular Cell Biology
Urs Ziegler: Center for Microscopy and Image Analysis, University of Zurich
Imre Berger: EMBL
Kurt Ballmer-Hofer: Paul Scherrer Institute, Biomolecular Research, Molecular Cell Biology
Philipp Berger: Paul Scherrer Institute, Biomolecular Research, Molecular Cell Biology

Nature Communications, 2010, vol. 1, issue 1, 1-6

Abstract: Abstract The introduction of heterologous genetic information, particularly of multiple genes, into mammalian cells is a key technology in contemporary experimental biological research. The coexpression of fluorescently tagged sensors is required to simultaneously analyse multiple parameters in living cells and the coexpression of several proteins is necessary to manipulate cell fate in stem cell biology. Current technologies for multigene expression in mammalian cells are inefficient, inflexible and time-consuming. In this paper we describe MultiLabel, a novel and highly efficient modular plasmid-based eukaryotic expression system. Independent expression vectors are assembled by a Cre/LoxP reaction into a plasmid with multiple expression cassettes. MultiLabel enables rapid construction of multigene expression vectors for the single-step creation of transiently or stably transfected mammalian cells.

Date: 2010
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DOI: 10.1038/ncomms1120

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