Herpesviruses carrying a Brainbow cassette reveal replication and expression of limited numbers of incoming genomes

Oren Kobiler, Yaron Lipman, Kate Therkelsen, Ingrid Daubechies and Lynn W. Enquist ()
Additional contact information
Oren Kobiler: Princeton University
Yaron Lipman: The Program in Applied and Computational Mathematics, Princeton University
Kate Therkelsen: The Program in Applied and Computational Mathematics, Princeton University
Ingrid Daubechies: The Program in Applied and Computational Mathematics, Princeton University
Lynn W. Enquist: Princeton University

Nature Communications, 2010, vol. 1, issue 1, 1-8

Abstract: Abstract Whether all the infectious herpesvirus particles entering a cell are able to replicate and/or express their genomes is not known. Here, we developed a general method to determine the number of viral genomes expressed in an infected cell. We constructed and analysed fluorophore expression from a recombinant pseudorabies virus (PRV263) carrying a Brainbow cassette (Cre-conditional expression of different fluorophores). Using three isogenic strains derived from PRV263, each expressing a single fluorophore, we analysed the colour composition of cells infected with these three viruses at different multiplicities. We estimate that fewer than seven incoming genomes are expressed per cell. In addition, those templates that are expressed are the genomes selected for replication and packaging into virions. This finite limit on the number of viral genomes that can be expressed is an intrinsic property of the infected cell and may be influenced by viral and cellular factors.

Date: 2010
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms1145 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:1:y:2010:i:1:d:10.1038_ncomms1145

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms1145

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().