Annexin-A5 assembled into two-dimensional arrays promotes cell membrane repair

Anthony Bouter, Céline Gounou, Rémi Bérat, Sisareuth Tan, Bernard Gallois, Thierry Granier, Béatrice Langlois d'Estaintot, Ernst Pöschl, Bent Brachvogel and Alain R. Brisson ()
Additional contact information
Anthony Bouter: Molecular Imaging and NanoBioTechnology, IECB, UMR-5248 CBMN CNRS-University Bordeaux1-ENITAB
Céline Gounou: Molecular Imaging and NanoBioTechnology, IECB, UMR-5248 CBMN CNRS-University Bordeaux1-ENITAB
Rémi Bérat: Molecular Imaging and NanoBioTechnology, IECB, UMR-5248 CBMN CNRS-University Bordeaux1-ENITAB
Sisareuth Tan: Molecular Imaging and NanoBioTechnology, IECB, UMR-5248 CBMN CNRS-University Bordeaux1-ENITAB
Bernard Gallois: Crystallography of Biological Macromolecules, UMR-5248 CBMN CNRS-University Bordeaux1-ENITAB
Thierry Granier: Crystallography of Biological Macromolecules, UMR-5248 CBMN CNRS-University Bordeaux1-ENITAB
Béatrice Langlois d'Estaintot: Crystallography of Biological Macromolecules, UMR-5248 CBMN CNRS-University Bordeaux1-ENITAB
Ernst Pöschl: School of Biological Sciences, University of East Anglia
Bent Brachvogel: Center for Biochemistry, Center for Molecular Medicine Cologne, Medical Faculty, University of Cologne
Alain R. Brisson: Molecular Imaging and NanoBioTechnology, IECB, UMR-5248 CBMN CNRS-University Bordeaux1-ENITAB

Nature Communications, 2011, vol. 2, issue 1, 1-9

Abstract: Abstract Eukaryotic cells possess a universal repair machinery that ensures rapid resealing of plasma membrane disruptions. Before resealing, the torn membrane is submitted to considerable tension, which functions to expand the disruption. Here we show that annexin-A5 (AnxA5), a protein that self-assembles into two-dimensional (2D) arrays on membranes upon Ca2+ activation, promotes membrane repair. Compared with wild-type mouse perivascular cells, AnxA5-null cells exhibit a severe membrane repair defect. Membrane repair in AnxA5-null cells is rescued by addition of AnxA5, which binds exclusively to disrupted membrane areas. In contrast, an AnxA5 mutant that lacks the ability of forming 2D arrays is unable to promote membrane repair. We propose that AnxA5 participates in a previously unrecognized step of the membrane repair process: triggered by the local influx of Ca2+, AnxA5 proteins bind to torn membrane edges and form a 2D array, which prevents wound expansion and promotes membrane resealing.

Date: 2011
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DOI: 10.1038/ncomms1270

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