Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells

María Mittelbrunn, Cristina Gutiérrez-Vázquez, Carolina Villarroya-Beltri, Susana González, Fátima Sánchez-Cabo, Manuel Ángel González, Antonio Bernad and Francisco Sánchez-Madrid ()
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María Mittelbrunn: Centro Nacional de Investigaciones Cardiovasculares, Melchor Fernández Almagro
Cristina Gutiérrez-Vázquez: Centro Nacional de Investigaciones Cardiovasculares, Melchor Fernández Almagro
Carolina Villarroya-Beltri: Centro Nacional de Investigaciones Cardiovasculares, Melchor Fernández Almagro
Susana González: Centro Nacional de Investigaciones Cardiovasculares, Melchor Fernández Almagro
Fátima Sánchez-Cabo: Centro Nacional de Investigaciones Cardiovasculares, Melchor Fernández Almagro
Manuel Ángel González: Centro Nacional de Investigaciones Cardiovasculares, Melchor Fernández Almagro
Antonio Bernad: Centro Nacional de Investigaciones Cardiovasculares, Melchor Fernández Almagro
Francisco Sánchez-Madrid: Centro Nacional de Investigaciones Cardiovasculares, Melchor Fernández Almagro

Nature Communications, 2011, vol. 2, issue 1, 1-10

Abstract: Abstract The immune synapse is an exquisitely evolved means of communication between T cells and antigen-presenting cells (APCs) during antigen recognition. Recent evidence points to the transfer of RNA via exosomes as a novel mode of intercellular communication. Here we show that exosomes of T, B and dendritic immune cells contain microRNA (miRNA) repertoires that differ from those of their parent cells. We investigate whether miRNAs are exchanged during cognate immune interactions, and demonstrate the existence of antigen-driven unidirectional transfer of miRNAs from the T cell to the APC, mediated by the delivery of CD63+ exosomes on immune synapse formation. Inhibition of exosome production by targeting neutral sphingomyelinase-2 impairs transfer of miRNAs to APCs. Moreover, miRNAs transferred during immune synapsis are able to modulate gene expression in recipient cells. Thus, our results support a mechanism of cellular communication involving antigen-dependent, unidirectional intercellular transfer of miRNAs by exosomes during immune synapsis.

Date: 2011
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:2:y:2011:i:1:d:10.1038_ncomms1285

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DOI: 10.1038/ncomms1285

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