Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome

Jayachandran Gopalakrishnan, Vito Mennella, Stephanie Blachon, Bo Zhai, Andrew H. Smith, Timothy L. Megraw, Daniela Nicastro, Steven P. Gygi, David A. Agard and Tomer Avidor-Reiss ()
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Jayachandran Gopalakrishnan: Harvard Medical School
Vito Mennella: University of California
Stephanie Blachon: Harvard Medical School
Bo Zhai: Harvard Medical School
Andrew H. Smith: Harvard Medical School
Timothy L. Megraw: College of Medicine, Florida State University
Daniela Nicastro: Brandeis University
Steven P. Gygi: Harvard Medical School
David A. Agard: University of California
Tomer Avidor-Reiss: Harvard Medical School

Nature Communications, 2011, vol. 2, issue 1, 1-11

Abstract: Abstract Centrosomes are conserved organelles that are essential for accurate cell division and cilium formation. A centrosome consists of a pair of centrioles surrounded by a protein network of pericentriolar material (PCM) that is essential for the centrosome's function. In this study, we show that Sas-4 provides a scaffold for cytoplasmic complexes (named S-CAP), which include CNN, Asl and D-PLP, proteins that are all found in the centrosomes at the vicinity of the centriole. When Sas-4 is absent, nascent procentrioles are unstable and lack PCM, and functional centrosomes are not generated. When Sas-4 is mutated, so that it cannot form S-CAP complexes, centrosomes are present but with dramatically reduced levels of PCM. Finally, purified S-CAP complexes or recombinant Sas-4 can bind centrosomes stripped of PCM, whereas recombinant CNN or Asl cannot. In summary, PCM assembly begins in the cytosol where Sas-4 provides a scaffold for pre-assembled cytoplasmic complexes before tethering of the complexes in a centrosome.

Date: 2011
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DOI: 10.1038/ncomms1367

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