Smad6-specific recruitment of Smurf E3 ligases mediates TGF-β1-induced degradation of MyD88 in TLR4 signalling

Youn Sook Lee, Jin Seok Park, Jun Hwan Kim, Su Myung Jung, Jae Young Lee, Seong-Jin Kim and Seok Hee Park ()
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Youn Sook Lee: Sungkyunkwan University
Jin Seok Park: Sungkyunkwan University
Jun Hwan Kim: Sungkyunkwan University
Su Myung Jung: Sungkyunkwan University
Jae Young Lee: Sungkyunkwan University
Seong-Jin Kim: CHA Cancer Institute, CHA University
Seok Hee Park: Sungkyunkwan University

Nature Communications, 2011, vol. 2, issue 1, 1-11

Abstract: Abstract Transforming growth factor-β (TGF-β) is a potent anti-inflammatory cytokine that regulates interleukin-1 receptor and Toll-like receptor (TLR) signalling. Here we show a novel mechanism where TGF-β1-induced K48-linked polyubiquitination and degradation of the adaptor MyD88 protein is dependent on the Smad6 protein, but not Smad7, and mediated by recruitment of the Smad ubiquitin regulator factor proteins, Smurf1 and Smurf2, which have E3-ubiquitin ligase activity. Smurf1 interaction with MyD88 appears to be mediated by Smad6, and Smurf2 interaction by Smurf1. Knockdown of endogenous Smurf1 or Smurf2 by RNA interference significantly suppresses the anti-inflammatory effects of TGF-β1 by preventing lipopolysaccharide-induced NF-κB nuclear translocation, resulting in de-suppression of pro-inflammatory gene expression. Similar effects are observed on the lipoteichoic-acid-induced TLR2 pathway, which is also MyD88-dependent, but not the MyD88-independent TLR3 pathway. Thus, our results suggest that MyD88 degradation driven by the Smad6–Smurf pathway is a novel mechanism for TGF-β1-mediated negative regulation of MyD88-dependent pro-inflammatory signalling.

Date: 2011
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DOI: 10.1038/ncomms1469

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