Plasmonic substrates for multiplexed protein microarrays with femtomolar sensitivity and broad dynamic range

Scott M. Tabakman, Lana Lau, Joshua T. Robinson, Jordan Price, Sarah P. Sherlock, Hailiang Wang, Bo Zhang, Zhuo Chen, Stephanie Tangsombatvisit, Justin A. Jarrell, Paul J. Utz and Hongjie Dai ()
Additional contact information
Scott M. Tabakman: Stanford University, Stanford, California 94305, USA.
Lana Lau: Stanford University, Stanford, California 94305, USA.
Joshua T. Robinson: Stanford University, Stanford, California 94305, USA.
Jordan Price: Stanford University School of Medicine
Sarah P. Sherlock: Stanford University, Stanford, California 94305, USA.
Hailiang Wang: Stanford University, Stanford, California 94305, USA.
Bo Zhang: Stanford University, Stanford, California 94305, USA.
Zhuo Chen: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha 410082, China.
Stephanie Tangsombatvisit: Stanford University School of Medicine
Justin A. Jarrell: Stanford University School of Medicine
Paul J. Utz: Stanford University School of Medicine
Hongjie Dai: Stanford University, Stanford, California 94305, USA.

Nature Communications, 2011, vol. 2, issue 1, 1-9

Abstract: Abstract Protein chips are widely used for high-throughput proteomic analysis, but to date, the low sensitivity and narrow dynamic range have limited their capabilities in diagnostics and proteomics. Here we present protein microarrays on a novel nanostructured, plasmonic gold film with near-infrared fluorescence enhancement of up to 100-fold, extending the dynamic range of protein detection by three orders of magnitude towards the fM regime. We employ plasmonic protein microarrays for the early detection of a cancer biomarker, carcinoembryonic antigen, in the sera of mice bearing a xenograft tumour model. Further, we demonstrate a multiplexed autoantigen array for human autoantibodies implicated in a range of autoimmune diseases with superior signal-to-noise ratios and broader dynamic range compared with commercial nitrocellulose and glass substrates. The high sensitivity, broad dynamic range and easy adaptability of plasmonic protein chips presents new opportunities in proteomic research and diagnostics applications.

Date: 2011
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DOI: 10.1038/ncomms1477

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