Extrasynaptic vesicle recycling in mature hippocampal neurons

Arjuna Ratnayaka, Vincenzo Marra, Tiago Branco and Kevin Staras ()
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Arjuna Ratnayaka: School of Life Sciences, University of Sussex
Vincenzo Marra: School of Life Sciences, University of Sussex
Tiago Branco: Wolfson Institute for Biomedical Research, University College London, London WC1E 6BT, UK.
Kevin Staras: School of Life Sciences, University of Sussex

Nature Communications, 2011, vol. 2, issue 1, 1-11

Abstract: Abstract Fast neuronal signalling relies on highly regulated vesicle fusion and recycling at specialized presynaptic terminals. Recently, examples of non-classical neurotransmission have also been reported, where fusion of vesicles can occur at sites remote from conventional synapses. This has potentially broad biological implications, but the underlying mechanisms are not well established. Here we show that a complete vesicle recycling pathway can occur at discrete axonal sites in mature hippocampal neurons and that extrasynaptic fusion is a robust feature of native tissue. We demonstrate that laterally mobile vesicle clusters trafficking between synaptic terminals become transiently stabilized by evoked action potentials and undergo complete but delayed Ca2+-dependent fusion along axons. This fusion is associated with dynamic actin accumulation and, subsequently, vesicles can be locally recycled, re-acidified and re-used. Immunofluorescence and ultrastructural work demonstrates that extrasynaptic fusion sites can have apposed postsynaptic specializations, suggesting that mobile vesicle recycling may underlie highly dynamic neuron–neuron communication.

Date: 2011
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:2:y:2011:i:1:d:10.1038_ncomms1534

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DOI: 10.1038/ncomms1534

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