Functionalized arrays of Raman-enhancing nanoparticles for capture and culture-free analysis of bacteria in human blood

Ting-Yu Liu, Kun-Tong Tsai, Huai-Hsien Wang, Yu Chen, Yu-Hsuan Chen, Yuan-Chun Chao, Hsuan-Hao Chang, Chi-Hung Lin, Juen-Kai Wang and Yuh-Lin Wang ()
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Ting-Yu Liu: Institute of Atomic and Molecular Sciences
Kun-Tong Tsai: Institute of Atomic and Molecular Sciences
Huai-Hsien Wang: Institute of Atomic and Molecular Sciences
Yu Chen: Institute of Atomic and Molecular Sciences
Yu-Hsuan Chen: Institute of Atomic and Molecular Sciences
Yuan-Chun Chao: Institute of Atomic and Molecular Sciences
Hsuan-Hao Chang: Institute of Atomic and Molecular Sciences
Chi-Hung Lin: Institute of Microbiology and Immunology, School of Life Science, National Yang-Ming University
Juen-Kai Wang: Institute of Atomic and Molecular Sciences
Yuh-Lin Wang: Institute of Atomic and Molecular Sciences

Nature Communications, 2011, vol. 2, issue 1, 1-8

Abstract: Abstract Detecting bacteria in clinical samples without using time-consuming culture processes would allow rapid diagnoses. Such a culture-free detection method requires the capture and analysis of bacteria from a body fluid, which are usually of complicated composition. Here we show that coating Ag-nanoparticle arrays with vancomycin (Van) can provide label-free analysis of bacteria via surface-enhanced Raman spectroscopy (SERS), leading to a ~1,000-fold increase in bacteria capture, without introducing significant spectral interference. Bacteria from human blood can be concentrated onto a microscopic Van-coated area while blood cells are excluded. Furthermore, a Van-coated substrate provides distinctly different SERS spectra of Van-susceptible and Van-resistant Enterococcus, indicating its potential use for drug-resistance tests. Our results represent a critical step towards the creation of SERS-based multifunctional biochips for rapid culture- and label-free detection and drug-resistant testing of microorganisms in clinical samples.

Date: 2011
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DOI: 10.1038/ncomms1546

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