Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropsis gaditana

Randor Radakovits, Robert E. Jinkerson, Susan I. Fuerstenberg, Hongseok Tae, Robert E. Settlage, Jeffrey L. Boore and Matthew C. Posewitz ()
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Randor Radakovits: Colorado School of Mines
Robert E. Jinkerson: Colorado School of Mines
Susan I. Fuerstenberg: Genome Project Solutions
Hongseok Tae: Data Analysis Core, Virginia Bioinformatics Institute, Virginia Tech
Robert E. Settlage: Data Analysis Core, Virginia Bioinformatics Institute, Virginia Tech
Jeffrey L. Boore: Genome Project Solutions
Matthew C. Posewitz: Colorado School of Mines

Nature Communications, 2012, vol. 3, issue 1, 1-11

Abstract: Abstract The potential use of algae in biofuels applications is receiving significant attention. However, none of the current algal model species are competitive production strains. Here we present a draft genome sequence and a genetic transformation method for the marine microalga Nannochloropsis gaditana CCMP526. We show that N. gaditana has highly favourable lipid yields, and is a promising production organism. The genome assembly includes nuclear (~29 Mb) and organellar genomes, and contains 9,052 gene models. We define the genes required for glycerolipid biogenesis and detail the differential regulation of genes during nitrogen-limited lipid biosynthesis. Phylogenomic analysis identifies genetic attributes of this organism, including unique stramenopile photosynthesis genes and gene expansions that may explain the distinguishing photoautotrophic phenotypes observed. The availability of a genome sequence and transformation methods will facilitate investigations into N. gaditana lipid biosynthesis and permit genetic engineering strategies to further improve this naturally productive alga.

Date: 2012
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DOI: 10.1038/ncomms1688

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