Intracellular temperature mapping with a fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy

Kohki Okabe, Noriko Inada, Chie Gota, Yoshie Harada, Takashi Funatsu and Seiichi Uchiyama ()
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Kohki Okabe: Graduate School of Pharmaceutical Sciences, The University of Tokyo
Noriko Inada: The Graduate School of Biological Sciences, Nara Institute of Science and Technology
Chie Gota: Graduate School of Pharmaceutical Sciences, The University of Tokyo
Yoshie Harada: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Honmachi Sakyo-ku
Takashi Funatsu: Graduate School of Pharmaceutical Sciences, The University of Tokyo
Seiichi Uchiyama: Graduate School of Pharmaceutical Sciences, The University of Tokyo

Nature Communications, 2012, vol. 3, issue 1, 1-9

Abstract: Abstract Cellular functions are fundamentally regulated by intracellular temperature, which influences biochemical reactions inside a cell. Despite the important contributions to biological and medical applications that it would offer, intracellular temperature mapping has not been achieved. Here we demonstrate the first intracellular temperature mapping based on a fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy. The spatial and temperature resolutions of our thermometry were at the diffraction limited level (200 nm) and 0.18–0.58 °C. The intracellular temperature distribution we observed indicated that the nucleus and centrosome of a COS7 cell, both showed a significantly higher temperature than the cytoplasm and that the temperature gap between the nucleus and the cytoplasm differed depending on the cell cycle. The heat production from mitochondria was also observed as a proximal local temperature increase. These results showed that our new intracellular thermometry could determine an intrinsic relationship between the temperature and organelle function.

Date: 2012
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DOI: 10.1038/ncomms1714

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