Hydroxylation of 5-methylcytosine by TET2 maintains the active state of the mammalian HOXA cluster

Michael T. Bocker, Francesca Tuorto, Günter Raddatz, Tanja Musch, Feng-Chun Yang, Mingjiang Xu, Frank Lyko and Achim Breiling ()
Additional contact information
Michael T. Bocker: DKFZ-ZMBH Alliance, German Cancer Research Center
Francesca Tuorto: DKFZ-ZMBH Alliance, German Cancer Research Center
Günter Raddatz: DKFZ-ZMBH Alliance, German Cancer Research Center
Tanja Musch: DKFZ-ZMBH Alliance, German Cancer Research Center
Feng-Chun Yang: Herman B Wells Center for Pediatric Research, Indiana University Melvin and Bren Simon Cancer Center, Indiana University School of Medicine
Mingjiang Xu: Herman B Wells Center for Pediatric Research, Indiana University Melvin and Bren Simon Cancer Center, Indiana University School of Medicine
Frank Lyko: DKFZ-ZMBH Alliance, German Cancer Research Center
Achim Breiling: DKFZ-ZMBH Alliance, German Cancer Research Center

Nature Communications, 2012, vol. 3, issue 1, 1-12

Abstract: Abstract Differentiation is accompanied by extensive epigenomic reprogramming, leading to the repression of stemness factors and the transcriptional maintenance of activated lineage-specific genes. Here we use the mammalian Hoxa cluster of developmental genes as a model system to follow changes in DNA modification patterns during retinoic acid-induced differentiation. We find the inactive cluster to be marked by defined patterns of 5-methylcytosine (5mC). Upon the induction of differentiation, the active anterior part of the cluster becomes increasingly enriched in 5-hydroxymethylcytosine (5hmC), following closely the colinear activation pattern of the gene array, which is paralleled by the reduction of 5mC. Depletion of the 5hmC generating dioxygenase Tet2 impairs the maintenance of Hoxa activity and partially restores 5mC levels. Our results indicate that gene-specific 5mC–5hmC conversion by Tet2 is crucial for the maintenance of active chromatin states at lineage-specific loci.

Date: 2012
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/ncomms1826 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms1826

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms1826

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().