High-performance and site-directed in utero electroporation by a triple-electrode probe

Marco dal Maschio, Diego Ghezzi, Guillaume Bony, Alessandro Alabastri, Gabriele Deidda, Marco Brondi, Sebastian Sulis Sato, Remo Proietti Zaccaria, Enzo Di Fabrizio, Gian Michele Ratto and Laura Cancedda ()
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Marco dal Maschio: Istituto Italiano di Tecnologia
Diego Ghezzi: Istituto Italiano di Tecnologia
Guillaume Bony: Istituto Italiano di Tecnologia
Alessandro Alabastri: Istituto Italiano di Tecnologia
Gabriele Deidda: Istituto Italiano di Tecnologia
Marco Brondi: Laboratorio NEST, Istituto Nanoscienze CNR and Scuola Normale Superiore
Sebastian Sulis Sato: Laboratorio NEST, Istituto Nanoscienze CNR and Scuola Normale Superiore
Remo Proietti Zaccaria: Istituto Italiano di Tecnologia
Enzo Di Fabrizio: Istituto Italiano di Tecnologia
Gian Michele Ratto: Laboratorio NEST, Istituto Nanoscienze CNR and Scuola Normale Superiore
Laura Cancedda: Istituto Italiano di Tecnologia

Nature Communications, 2012, vol. 3, issue 1, 1-11

Abstract: Abstract In utero electroporation is a powerful tool to transfect and manipulate neural-precursor cells of the rodent parietal cortex and their progeny in vivo. Although this technique can potentially target numerous brain areas, reliability of transfection in some brain regions is low or physical access is limited. Here we present a new in utero electroporation configuration based on the use of three electrodes, the relative position and polarities of which can be adjusted. The technique allows easy access and exceedingly reliable monolateral or bilateral transfection at brain locations that could only be sporadically targeted before. By improvement in the efficiency of the electrical field distribution, demonstrated here by a mathematical simulation, the multi-electrode configuration also extends the developmental timeframe for reliable in utero electroporation, allowing for the first time specific transfection of Purkinje cells in the rat cerebellum.

Date: 2012
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DOI: 10.1038/ncomms1961

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