Biocompatibility of a genetically encoded calcium indicator in a transgenic mouse model

Stephan Direnberger, Marsilius Mues, Vincenzo Micale, Carsten T. Wotjak, Steffen Dietzel, Michael Schubert, Andreas Scharr, Sami Hassan, Christian Wahl-Schott, Martin Biel, Gurumoorthy Krishnamoorthy and Oliver Griesbeck ()
Additional contact information
Stephan Direnberger: Max-Planck-Institut für Neurobiologie
Marsilius Mues: Max-Planck-Institut für Neurobiologie
Vincenzo Micale: Max-Planck-Institut für Psychiatrie
Carsten T. Wotjak: Max-Planck-Institut für Psychiatrie
Steffen Dietzel: Walter-Brendel-Zentrum für experimentelle Medizin, Ludwig-Maximilians-Universität München
Michael Schubert: Walter-Brendel-Zentrum für experimentelle Medizin, Ludwig-Maximilians-Universität München
Andreas Scharr: Center for Integrated Protein Science (CIPS) and Zentrum für Pharmaforschung, Ludwig-Maximilians-Universität München
Sami Hassan: Center for Integrated Protein Science (CIPS) and Zentrum für Pharmaforschung, Ludwig-Maximilians-Universität München
Christian Wahl-Schott: Center for Integrated Protein Science (CIPS) and Zentrum für Pharmaforschung, Ludwig-Maximilians-Universität München
Martin Biel: Center for Integrated Protein Science (CIPS) and Zentrum für Pharmaforschung, Ludwig-Maximilians-Universität München
Gurumoorthy Krishnamoorthy: Max-Planck-Institut für Neurobiologie
Oliver Griesbeck: Max-Planck-Institut für Neurobiologie

Nature Communications, 2012, vol. 3, issue 1, 1-10

Abstract: Abstract Engineering efforts of genetically encoded calcium indicators predominantly focused on enhancing fluorescence changes, but how indicator expression affects the physiology of host organisms is often overlooked. Here, we demonstrate biocompatibility and widespread functional expression of the genetically encoded calcium indicator TN-XXL in a transgenic mouse model. To validate the model and characterize potential effects of indicator expression we assessed both indicator function and a variety of host parameters, such as anatomy, physiology, behaviour and gene expression profiles in these mice. We also demonstrate the usefulness of primary cells and organ explants prepared from these mice for imaging applications. Although we find mild signatures of indicator expression that may be further reduced in future sensor generations, the 'green' indicator mice generated provide a well-characterized resource of primary cells and tissues for in vitro and in vivo calcium imaging applications.

Date: 2012
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms2035 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms2035

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms2035

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().