Real-time in vivo imaging of the beating mouse heart at microscopic resolution

Sungon Lee, Claudio Vinegoni (), Paolo Fumene Feruglio, Lyuba Fexon, Rostic Gorbatov, Misha Pivoravov, Andrea Sbarbati, Matthias Nahrendorf and Ralph Weissleder
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Sungon Lee: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center
Claudio Vinegoni: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center
Paolo Fumene Feruglio: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center
Lyuba Fexon: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center
Rostic Gorbatov: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center
Misha Pivoravov: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center
Andrea Sbarbati: Neuropsychological, Morphological and Movement Sciences, University of Verona
Matthias Nahrendorf: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center
Ralph Weissleder: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center

Nature Communications, 2012, vol. 3, issue 1, 1-8

Abstract: Abstract Real-time imaging of moving organs and tissues at microscopic resolutions represents a major challenge in studying the complex biology of live animals. Here we present a technique based on a novel stabilizer setup combined with a gating acquisition algorithm for the imaging of a beating murine heart at the single-cell level. The method allows serial in vivo fluorescence imaging of the beating heart in live mice in both confocal and nonlinear modes over the course of several hours. We demonstrate the utility of this technique for in vivo optical sectioning and dual-channel time-lapse fluorescence imaging of cardiac ischaemia. The generic method could be adapted to other moving organs and thus broadly facilitate in vivo microscopic investigations.

Date: 2012
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DOI: 10.1038/ncomms2060

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