Direct visualization of cell division using high-resolution imaging of M-phase of the cell cycle

Michael Hesse, Alexandra Raulf, Gregor-Alexander Pilz, Christian Haberlandt, Alexandra M. Klein, Ronald Jabs, Holm Zaehres, Christopher J. Fügemann, Katrin Zimmermann, Jonel Trebicka, Armin Welz, Alexander Pfeifer, Wilhelm Röll, Michael I. Kotlikoff, Christian Steinhäuser, Magdalena Götz, Hans R. Schöler and Bernd K. Fleischmann ()
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Michael Hesse: Institute of Physiology I, Life and Brain Center, University of Bonn
Alexandra Raulf: Institute of Physiology I, Life and Brain Center, University of Bonn
Gregor-Alexander Pilz: Institute of Stem Cell Research
Christian Haberlandt: Institute of Cellular Neurosciences, University of Bonn
Alexandra M. Klein: Institute of Physiology I, Life and Brain Center, University of Bonn
Ronald Jabs: Institute of Cellular Neurosciences, University of Bonn
Holm Zaehres: Max Planck Institute for Molecular Biomedicine
Christopher J. Fügemann: Institute of Physiology I, Life and Brain Center, University of Bonn
Katrin Zimmermann: Institute of Pharmacology and Toxicology, University of Bonn
Jonel Trebicka: University of Bonn
Armin Welz: University of Bonn
Alexander Pfeifer: Institute of Pharmacology and Toxicology, University of Bonn
Wilhelm Röll: University of Bonn
Michael I. Kotlikoff: College of Veterinary Medicine, Cornell University
Christian Steinhäuser: Institute of Cellular Neurosciences, University of Bonn
Magdalena Götz: Institute of Stem Cell Research
Hans R. Schöler: Max Planck Institute for Molecular Biomedicine
Bernd K. Fleischmann: Institute of Physiology I, Life and Brain Center, University of Bonn

Nature Communications, 2012, vol. 3, issue 1, 1-12

Abstract: Abstract Current approaches to monitor and quantify cell division in live cells, and reliably distinguish between acytokinesis and endoreduplication, are limited and complicate determination of stem cell pool identities. Here we overcome these limitations by generating an in vivo reporter system using the scaffolding protein anillin fused to enhanced green fluorescent protein, to provide high spatiotemporal resolution of mitotic phase. This approach visualizes cytokinesis and midbody formation as hallmarks of expansion of stem and somatic cells, and enables distinction from cell cycle variations. High-resolution microscopy in embryonic heart and brain tissues of enhanced green fluorescent protein–anillin transgenic mice allows live monitoring of cell division and quantitation of cell cycle kinetics. Analysis of cell division in hearts post injury shows that border zone cardiomyocytes in the infarct respond with increasing ploidy, but not cell division. Thus, the enhanced green fluorescent protein–anillin system enables monitoring and measurement of cell division in vivo and markedly simplifies in vitro analysis in fixed cells.

Date: 2012
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DOI: 10.1038/ncomms2089

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