Dynamic blastomere behaviour reflects human embryo ploidy by the four-cell stage

Shawn L. Chavez, Kevin E. Loewke, Jinnuo Han, Farshid Moussavi, Pere Colls, Santiago Munne, Barry Behr and Renee A. Reijo Pera ()
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Shawn L. Chavez: Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine
Kevin E. Loewke: Auxogyn, Inc.
Jinnuo Han: Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine
Farshid Moussavi: Auxogyn, Inc.
Pere Colls: Reprogenetics
Santiago Munne: Reprogenetics
Barry Behr: Stanford University School of Medicine
Renee A. Reijo Pera: Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine

Nature Communications, 2012, vol. 3, issue 1, 1-12

Abstract: Abstract Previous studies have demonstrated that aneuploidy in human embryos is surprisingly frequent with 50–80% of cleavage-stage human embryos carrying an abnormal chromosome number. Here we combine non-invasive time-lapse imaging with karyotypic reconstruction of all blastomeres in four-cell human embryos to address the hypothesis that blastomere behaviour may reflect ploidy during the first two cleavage divisions. We demonstrate that precise cell cycle parameter timing is observed in all euploid embryos to the four-cell stage, whereas only 30% of aneuploid embryos exhibit parameter values within normal timing windows. Further, we observe that the generation of human embryonic aneuploidy is complex with contribution from chromosome-containing fragments/micronuclei that frequently emerge and may persist or become reabsorbed during interphase. These findings suggest that cell cycle and fragmentation parameters of individual blastomeres are diagnostic of ploidy, amenable to automated tracking algorithms, and likely of clinical relevance in reducing transfer of embryos prone to miscarriage.

Date: 2012
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DOI: 10.1038/ncomms2249

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